The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

IME2  -  Ime2p

Saccharomyces cerevisiae S288c

Synonyms: J0817, Meiosis induction protein kinase IME2/SME1, SME1, YJL106W
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of IME2

  • We have created baculovirus expression vectors to produce recombinant Ime2 in insect cells [1].
 

High impact information on IME2

  • Regulation of meiotic S phase by Ime2 and a Clb5,6-associated kinase in Saccharomyces cerevisiae [2].
  • In accordance with a late role for Ime2, we observed an increase in its activity during M phase that depended on Cdc28 and Ndt80 [3].
  • Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2 [3].
  • The requirement of Ime2 for M phase is partially explained by its stimulation of the key meiotic transcription factor Ndt80, which is needed in turn for high Cdc28 activity [3].
  • Late addition of inhibitor to ime2-as1 revealed unexpected roles of Ime2 in the initiation and execution of chromosome segregation [3].
 

Biological context of IME2

  • Further investigation shows that during wild-type meiosis the IME2 promoter undergoes an increase in the level of bound acetylated histone H3 [4].
  • The expression of IME2 in cycling cells inhibited bud formation and caused cells to arrest in mitosis [5].
  • GCN5-dependent histone H3 acetylation and RPD3-dependent histone H4 deacetylation have distinct, opposing effects on IME2 transcription, during meiosis and during vegetative growth, in budding yeast [4].
  • Addition of moderate concentrations of glucose (0.5%) to sporulation medium did not inhibit transcription of two key activators of sporulation, IME1 and IME2, but did increase levels of Sic1p, a cyclin-dependent kinase inhibitor, resulting in a block to meiotic DNA replication [6].
  • We report here an analysis of the regulatory region of IME2, an IME1-dependent meiotic gene [7].
 

Anatomical context of IME2

  • Hypothesizing that genes important in meiotic processes in mammals might have evolutionarily conserved counterparts in lower organisms, we used the yeast IME2 meiotic gene (serine threonine kinase) as a probe for screening a mouse testis cDNA library [8].
 

Associations of IME2 with chemical compounds

  • Our data show that Cak1p is required to activate Ime2p through a mechanism that requires threonine 242 and tyrosine 244 in Ime2p's activation loop [9].
  • Ime1 is a transcriptional activator that is required for transcription of IME2, a serine/threonine protein kinase [10].
  • The yeast trimeric guanine nucleotide-binding protein alpha subunit, Gpa2p, controls the meiosis-specific kinase Ime2p activity in response to nutrients [11].
  • The carboxyl residue adjacent to the phosphoacceptor (+1 position) also influences the efficiency of Ime2 phosphorylation with alanine being a preferred residue [12].
 

Enzymatic interactions of IME2

 

Regulatory relationships of IME2

  • This suggests that Ime2 activates expression of NDT80 by eliminating Sum1-mediated repression [15].
  • We found that the multicopy of YPH1 not only suppressed slow growth but also decreased IME2 expression in the yvh1 disruptant [16].
  • Accordingly, we propose that the IME1 product stimulates meiosis mainly through activation of IME2 expression [17].
  • Sic1p is negatively regulated by Ime2p kinase, and several observations indicate that glucose inhibits meiotic DNA replication through SCF(Grr1p)-mediated destruction of this kinase [6].
  • The addition of glucose did not further inhibit IME2 transcription, suggesting that Snf1 is the primary mediator of glucose controls on IME2 expression [18].
 

Other interactions of IME2

  • This mutant also fails to induce IME2 transcription; IME1 transcription, however, is essentially normal [4].
  • We report that Gcn5, a histone H3 acetylase, plays a central role in initiation of meiosis via effects on IME2 expression [4].
  • Mutation of IME2 prevents expression of NDT80 in sporulating cells [15].
  • In meiosis, Sic1p destruction is signaled by the meiosis-specific Ime2p protein kinase [9].
  • Thus, UME6 and the URS1 site both have dual negative and positive roles at the IME2 UAS [7].
  • We propose that Ime2-dependent phosphorylation of a subset of cell-cycle proteins limits the effects of Cdc14 in meiosis [19].
 

Analytical, diagnostic and therapeutic context of IME2

References

  1. Purification and some properties of Saccharomyces cerevisiae meiosis-specific protein kinase Ime2. Hui, C.M., Campistrous, A., Stuart, D.T. Protein Expr. Purif. (2002) [Pubmed]
  2. Regulation of meiotic S phase by Ime2 and a Clb5,6-associated kinase in Saccharomyces cerevisiae. Dirick, L., Goetsch, L., Ammerer, G., Byers, B. Science (1998) [Pubmed]
  3. Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2. Benjamin, K.R., Zhang, C., Shokat, K.M., Herskowitz, I. Genes Dev. (2003) [Pubmed]
  4. GCN5-dependent histone H3 acetylation and RPD3-dependent histone H4 deacetylation have distinct, opposing effects on IME2 transcription, during meiosis and during vegetative growth, in budding yeast. Burgess, S.M., Ajimura, M., Kleckner, N. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  5. Inhibition of APC-mediated proteolysis by the meiosis-specific protein kinase Ime2. Bolte, M., Steigemann, P., Braus, G.H., Irniger, S. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  6. Glucose inhibits meiotic DNA replication through SCFGrr1p-dependent destruction of Ime2p kinase. Purnapatre, K., Gray, M., Piccirillo, S., Honigberg, S.M. Mol. Cell. Biol. (2005) [Pubmed]
  7. Bipartite structure of an early meiotic upstream activation sequence from Saccharomyces cerevisiae. Bowdish, K.S., Mitchell, A.P. Mol. Cell. Biol. (1993) [Pubmed]
  8. Tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts. Shalom, S., Don, J. Mol. Reprod. Dev. (1999) [Pubmed]
  9. The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p. Schindler, K., Benjamin, K.R., Martin, A., Boglioli, A., Herskowitz, I., Winter, E. Mol. Cell. Biol. (2003) [Pubmed]
  10. Ime2, a meiosis-specific kinase in yeast, is required for destabilization of its transcriptional activator, Ime1. Guttmann-Raviv, N., Martin, S., Kassir, Y. Mol. Cell. Biol. (2002) [Pubmed]
  11. The yeast trimeric guanine nucleotide-binding protein alpha subunit, Gpa2p, controls the meiosis-specific kinase Ime2p activity in response to nutrients. Donzeau, M., Bandlow, W. Mol. Cell. Biol. (1999) [Pubmed]
  12. Arg-Pro-X-Ser/Thr Is a Consensus Phosphoacceptor Sequence for the Meiosis-Specific Ime2 Protein Kinase in Saccharomyces cerevisiae. Moore, M., Shin, M.E., Bruning, A., Schindler, K., Vershon, A., Winter, E. Biochemistry (2007) [Pubmed]
  13. Purification and characterization of the DNA binding domain of Saccharomyces cerevisiae meiosis-specific transcription factor Ndt80. Sopko, R., Stuart, D.T. Protein Expr. Purif. (2004) [Pubmed]
  14. Saccharomyces cerevisiae Ime2 phosphorylates Sic1 at multiple PXS/T sites but is insufficient to trigger Sic1 degradation. Sedgwick, C., Rawluk, M., Decesare, J., Raithatha, S., Wohlschlegel, J., Semchuk, P., Ellison, M., Yates, J., Stuart, D. Biochem. J. (2006) [Pubmed]
  15. Regulation of the premiddle and middle phases of expression of the NDT80 gene during sporulation of Saccharomyces cerevisiae. Pak, J., Segall, J. Mol. Cell. Biol. (2002) [Pubmed]
  16. Dual-specificity protein phosphatase Yvh1p, which is required for vegetative growth and sporulation, interacts with yeast pescadillo homolog in Saccharomyces cerevisiae. Sakumoto, N., Yamashita, H., Mukai, Y., Kaneko, Y., Harashima, S. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  17. A transcriptional cascade governs entry into meiosis in Saccharomyces cerevisiae. Smith, H.E., Mitchell, A.P. Mol. Cell. Biol. (1989) [Pubmed]
  18. Snf1 kinase connects nutritional pathways controlling meiosis in Saccharomyces cerevisiae. Honigberg, S.M., Lee, R.H. Mol. Cell. Biol. (1998) [Pubmed]
  19. Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis. Holt, L.J., Hutti, J.E., Cantley, L.C., Morgan, D.O. Mol. Cell (2007) [Pubmed]
  20. Genetic and molecular analysis of REC114, an early meiotic recombination gene in yeast. Pittman, D., Lu, W., Malone, R.E. Curr. Genet. (1993) [Pubmed]
  21. Protein kinase activity associated with the IME2 gene product, a meiotic inducer in the yeast Saccharomyces cerevisiae. Kominami, K., Sakata, Y., Sakai, M., Yamashita, I. Biosci. Biotechnol. Biochem. (1993) [Pubmed]
 
WikiGenes - Universities