Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

PubChem1322 2-amino-5-fluoro-benzoic acid

Synonyms: SureCN135091, AG-C-90587, ACMC-209jyo, ALBB-006133, ANW-30142, ...

Cheon, S.A. et al., Lebel, K. et al., Toyn, J.H. et al.

Toyn, Gunyuzlu, White, Thompson, Hollis,

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High impact information on NSC513308

Upon integration into the genome, these markers can be removed by the use of 5-fluoroorotic acid (URA3), 5-fluoroanthranilic acid (TRP1) or the FLP recombinase (MPA(R)) [1].
Since YlTRP1 could be used for counterselection in medium containing 5-fluoroanthranilic acid (5-FAA), we constructed TRP blasters that contained YlTRP1 flanked by a direct repeat of a sequence and allowed the recycling of the YlTRP1 marker [2].

Gene context of NSC513308

Described here is the use of 5-fluoroanthranilic acid for the counterselection of TRP1, a commonly used genetic marker in the yeast Saccharomyces cerevisiae [3].

References

New tools for phenotypic analysis in Candida albicans: the WAR1 gene confers resistance to sorbate. Lebel, K., MacPherson, S., Turcotte, B. Yeast (2006) [Pubmed]
Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster. Cheon, S.A., Han, E.J., Kang, H.A., Ogrydziak, D.M., Kim, J.Y. Yeast (2003) [Pubmed]
A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance. Toyn, J.H., Gunyuzlu, P.L., White, W.H., Thompson, L.A., Hollis, G.F. Yeast (2000) [Pubmed]

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