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Gene Review

TRP1  -  phosphoribosylanthranilate isomerase TRP1

Saccharomyces cerevisiae S288c

Synonyms: N-(5'-phosphoribosyl)anthranilate isomerase, PRAI, YDR007W
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Disease relevance of TRP1


High impact information on TRP1

  • Efficient repair also occurred in both strands of a disrupted TRP1 gene (ten PD sites), containing four unstable nucleosomes, and in a nucleosome gap at the 5' end of URA3 (two PD sites) [6].
  • This site lies at the 3' end of the TRP1 gene, in a region devoid of nucleosomes, and is positioned 80 bp away from the ARS consensus sequence; removal of this region impairs ARS function in vivo [7].
  • The fusion comprised the prepro region of prepro-alpha-factor (MF alpha 1) N-terminal to phosphoribosyl anthranilate isomerase (TRP1) [8].
  • This finding is confirmed by sequences around the C. glabrata TRP1 and IPP1 loci, which show that it contains sister regions derived from the same duplication event as that of S. cerevisiae [9].
  • A multicopy yeast plasmid containing the TRP1 gene (coding for N-5'-phosphoribosylanthranilate isomerase) and ARS1 (autonomously replicating sequence 1) has been purified as chromatin [10].

Chemical compound and disease context of TRP1


Biological context of TRP1


Anatomical context of TRP1

  • TRP1 was not phenotypically expressed within the organelle [17].
  • The generated split-Trp protein sensors allow for the detection of protein-protein interactions in the cytosol as well as the membrane by enabling trp1 cells to grow on medium lacking tryptophan [18].

Associations of TRP1 with chemical compounds


Physical interactions of TRP1

  • One was overlapping the ABF1 binding site on ARS1 and another protected region was found upstream to the translational start codon of the TRP1 gene [22].

Enzymatic interactions of TRP1

  • A diploid strain of the yeast Saccharomyces cerevisiae has been constructed that has one copy of the ribosomal protein gene CYH2 completely deleted and replaced with the TRP1 gene using the method of Rothstein (1983) [23].

Regulatory relationships of TRP1

  • To determine whether the DNA synthesis associated with repair of DSBs has a higher error rate than that associated with genome duplication, HO-induced DSBs were generated 0.3 kb from revertible alleles of trp1 [24].
  • By placing the subcloned N-TRP1 gene under the control of the inducible PHO5 promoter from yeast, the expression of all three activities was increased to more than ten fold that of wild-type yeast and the overproduced protein could be visualized by SDS-polyacrylamide gel electrophoresis of crude extract and Coomassie Blue staining [25].

Other interactions of TRP1


Analytical, diagnostic and therapeutic context of TRP1

  • A series of one-sided and internal deletions were constructed in vitro throughout the TRP1 promoter, and the effect of each deletion on transcription was assessed by Northern blotting [30].
  • One of the most versatile sets of PCR deletion/modification vectors is the pFA system described by Longtine et al.(1998), which is based on both heterologous (kanMX6 and HIS3MX6) and homologous (TRP1) markers [31].
  • To eliminate transfer artefacts, selection was first applied for the undamaged TRP1 gene followed by counter selection for URA3 gene activity, which indicated correct repair of the DSB and DSG [32].
  • Southern blot analysis indicated that HY contains five copies of the TRP1 gene [33].


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  7. Bent DNA at a yeast autonomously replicating sequence. Snyder, M., Buchman, A.R., Davis, R.W. Nature (1986) [Pubmed]
  8. In vivo and in vitro analysis of ptl1, a yeast ts mutant with a membrane-associated defect in protein translocation. Toyn, J., Hibbs, A.R., Sanz, P., Crowe, J., Meyer, D.I. EMBO J. (1988) [Pubmed]
  9. Gene order evolution and paleopolyploidy in hemiascomycete yeasts. Wong, S., Butler, G., Wolfe, K.H. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  10. Isolation of an episomal yeast gene and replication origin as chromatin. Pederson, D.S., Venkatesan, M., Thoma, F., Simpson, R.T. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  11. A cloned tryptophan-synthesis gene from the ascomycete Cochliobolus heterostrophus functions in Escherichia coli, yeast and Aspergillus nidulans. Turgeon, B.G., MacRae, W.D., Garber, R.C., Fink, G.R., Yoder, O.C. Gene (1986) [Pubmed]
  12. A series of yeast/Escherichia coli lambda expression vectors designed for directional cloning of cDNAs and cre/lox-mediated plasmid excision. Brunelli, J.P., Pall, M.L. Yeast (1993) [Pubmed]
  13. Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA. Zakian, V.A., Scott, J.F. Mol. Cell. Biol. (1982) [Pubmed]
  14. The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability. Garrett, J.M. Mol. Microbiol. (1997) [Pubmed]
  15. Fidelity of mitotic double-strand-break repair in Saccharomyces cerevisiae: a role for SAE2/COM1. Rattray, A.J., McGill, C.B., Shafer, B.K., Strathern, J.N. Genetics (2001) [Pubmed]
  16. Cloning and sequence analysis of the Pichia pastoris TRP1, IPP1 and HIS3 genes. Cosano, I., Alvarez, P., Molina, M., Nombela, C. Yeast (1998) [Pubmed]
  17. Nuclear mutations in Saccharomyces cerevisiae that affect the escape of DNA from mitochondria to the nucleus. Thorsness, P.E., Fox, T.D. Genetics (1993) [Pubmed]
  18. Transforming a (beta/alpha)8--barrel enzyme into a split-protein sensor through directed evolution. Tafelmeyer, P., Johnsson, N., Johnsson, K. Chem. Biol. (2004) [Pubmed]
  19. Cloning and sequence analysis of the TRP1 gene encoding the phosphoribosyl anthranilate isomerase from Pichia anomala (strain K). Friel, D., Vandenbol, M., Haïssam Jijakli, M. Yeast (2003) [Pubmed]
  20. A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance. Toyn, J.H., Gunyuzlu, P.L., White, W.H., Thompson, L.A., Hollis, G.F. Yeast (2000) [Pubmed]
  21. Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster. Cheon, S.A., Han, E.J., Kang, H.A., Ogrydziak, D.M., Kim, J.Y. Yeast (2003) [Pubmed]
  22. Characterization of DNA binding properties of Yp20: an abundant nuclear protein isolated from Saccharomyces cerevisiae. Bartuv, J., Valansi, C., Shalitin, C. Biochem. Biophys. Res. Commun. (1993) [Pubmed]
  23. Construction and characterization of a haploid strain of Saccharomyces cerevisiae that completely lacks all genomic CYH2 sequences. Miles, D.J., Donovan, D.M., Pearson, N.J. Curr. Genet. (1988) [Pubmed]
  24. DNA synthesis errors associated with double-strand-break repair. Strathern, J.N., Shafer, B.K., McGill, C.B. Genetics (1995) [Pubmed]
  25. Deletion analysis of domain independence in the TRP1 gene product of Neurospora crassa. Walker, M.S., DeMoss, J.A. Mol. Gen. Genet. (1990) [Pubmed]
  26. Low- and high-copy-number shuttle vectors for replication in the budding yeast Kluyveromyces lactis. Chen, X.J. Gene (1996) [Pubmed]
  27. Cloning of the Candida glabrata TRP1 and HIS3 genes, and construction of their disruptant strains by sequential integrative transformation. Kitada, K., Yamaguchi, E., Arisawa, M. Gene (1995) [Pubmed]
  28. Arrangement of genes TRP1 and TRP3 of Saccharomyces cerevisiae strains. Braus, G., Furter, R., Prantl, F., Niederberger, P., Hütter, R. Arch. Microbiol. (1985) [Pubmed]
  29. Designer deletion and prototrophic strains derived from Saccharomyces cerevisiae strain W303-1a. Replogle, K., Hovland, L., Rivier, D.H. Yeast (1999) [Pubmed]
  30. Multiple control elements in the TRP1 promoter of Saccharomyces cerevisiae. Kim, S., Mellor, J., Kingsman, A.J., Kingsman, S.M. Mol. Cell. Biol. (1986) [Pubmed]
  31. The trp1- delta FA designer deletion for PCR-based gene functional analysis in Saccharomyces cerevisiae. Horecka, J., Jigami, Y. Yeast (1999) [Pubmed]
  32. The use of a double-marker shuttle vector to study DNA double-strand break repair in wild-type and radiation-sensitive mutants of the yeast Saccharomyces cerevisiae. Jha, B., Ahne, F., Eckardt-Schupp, F. Curr. Genet. (1993) [Pubmed]
  33. Construction of a Trp- commercial baker's yeast strain by using food-safe-grade dominant drug resistance cassettes. Estruch, F., Prieto, J.A. FEMS Yeast Res. (2003) [Pubmed]
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