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Chemical Compound Review

Poly aug     [(2R,3S,4R,5R)-5-(2-amino-6- oxo-3H-purin-9...

Synonyms: KST-1A4434, KST-1A4435, Poly(A,U,G), AR-1A8150, AR-1A8151, ...
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Disease relevance of Poly aug

  • Binding and cross-linking of the uncharged, modified tRNAs to E. coli ribosomes have been studied with and without poly(A,U,G) as a message, under conditions directing uncharged tRNAs preferentially to the P-site [1].
  • In addition, initiation complex formation on uncapped cowpea mosaic virus RNA and on poly(A,U,G) was only slightly stimulated by ATP, much less than in the case of the capped RNAs [2].

High impact information on Poly aug

  • Purified IF3(mt) promotes initiation complex formation on mitochondrial 55 S ribosomes in the presence of mitochondrial initiation factor 2 (IF2(mt)), [(35)S]fMet-tRNA, and either poly(A,U,G) or an in vitro transcript of the cytochrome oxidase subunit II gene as mRNA [3].
  • In the presence of N-fMet-tRNAfMet and poly(A,U,G) or in the presence of N-acetyl-Phe-tRNAPhe and poly(U), initiation factor IF2 causes an additional decrease of the uncoupled EF-G GTPase activity [4].
  • In addition, phosphorylation of 40 S ribosomal subunits by the cAMP-dependent protein kinase inhibited translation of poly(A,U,G) by 30% in a reconstituted protein-synthesizing system [5].
  • Mammalian mitochondrial translational initiation factor 3 (IF3(mt)) promotes initiation complex formation on mitochondrial 55S ribosomes in the presence of IF2(mt), fMet-tRNA and poly(A,U,G) [6].

Anatomical context of Poly aug

  • The IF-2mt-mediated binding of fMet-tRNAi(Met) to mitochondrial ribosomes is dependent on the presence of a message such as poly(A,U,G) and on GTP [7].

Analytical, diagnostic and therapeutic context of Poly aug

  • The purification was monitored by Western immunoblotting and the activity of the purified factor was tested by measuring the stimulation of binding of the initiator fMet-tRNA(Met)f to 70S ribosomes in the presence of GTP and poly(A,U,G) as messenger RNA [8].


  1. Ribosomal proteins S7 and L1 are located close to the decoding site of E. coli ribosome--affinity labeling studies with modified tRNAs carrying photoreactive probes attached adjacent to the 3'-end of the anticodon. Podkowiński, J., Górnicki, P. Nucleic Acids Res. (1989) [Pubmed]
  2. The ATP requirement for initiation of eukaryotic translation varies according to the mRNA species. Jackson, R.J. Eur. J. Biochem. (1991) [Pubmed]
  3. Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs. Koc, E.C., Spremulli, L.L. J. Biol. Chem. (2002) [Pubmed]
  4. Regulation of elongation factor G GTPase activity by the ribosomal state. The effects of initiation factors and differentially bound tRNA, aminoacyl-tRNA, and peptidyl-tRNA. Voigt, J., Nagel, K. J. Biol. Chem. (1993) [Pubmed]
  5. Changes in ribosome function by cAMP-dependent and cAMP-independent phosphorylation of ribosomal protein S6. Burkhard, S.J., Traugh, J.A. J. Biol. Chem. (1983) [Pubmed]
  6. Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3. Bhargava, K., Spremulli, L.L. Nucleic Acids Res. (2005) [Pubmed]
  7. Identification and initial characterization of translational initiation factor 2 from bovine mitochondria. Liao, H.X., Spremulli, L.L. J. Biol. Chem. (1990) [Pubmed]
  8. Superexpression and fast purification of E coli initiation factor IF2. Mortensen, K.K., Nyengaard, N.R., Hershey, J.W., Laalami, S., Sperling-Petersen, H.U. Biochimie (1991) [Pubmed]
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