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MeSH Review

Blotting, Western

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Disease relevance of Blotting, Western


Psychiatry related information on Blotting, Western


High impact information on Blotting, Western


Chemical compound and disease context of Blotting, Western


Biological context of Blotting, Western


Anatomical context of Blotting, Western

  • Analysis of injected oocytes by Northern and Western blotting showed site-specific cleavage and subsequent degradation of the D7 mRNA and the failure of the D7 protein to accumulate during progesterone-induced maturation [26].
  • T cells stimulated by peptide-pulsed antigen-presenting cells (APCs) undergo an antigen dose-dependent decrease of the total cellular content of TCR-beta, CD3-epsilon, and zeta chains, as detected by FACS(R) analysis on fixed and permeabilized T-APC conjugates and by Western blot analysis on cell lysates [27].
  • Northern and Western blots revealed that inducible nitric oxide synthase (iNOS) mRNA and protein levels correlated directly with the ability of each macrophage population to produce NO, and the levels of these macromolecules were altered sufficiently in tumor bearers' macrophages to account for the diminished NO production described [28].
  • The expression of the leukocyte EL-246 antigen was regulated in the same manner as L-selectin and EL-246 recognized anti-L-selectin mAb affinity-purified antigen in SDS/PAGE Western blot analysis [29].
  • NH2-terminal sequence and/or Western blot analysis revealed the identity of two of the proteins as the endoplasmic reticulum (ER) resident stress proteins GRP94 and ERp72 [30].

Associations of Blotting, Western with chemical compounds

  • The same antibodies specifically react with a 400K protein in sodium dodecyl sulphate (SDS) extracts of normal human muscle subjected to Western blot analysis [31].
  • On a single determination, the latex agglutination slide test was found to be highly sensitive and specific compared with Western blot results in these populations with a relatively high prevalence of infection [32].
  • Anti-Fy6 reacted in western blots with a membrane glycoprotein of approximately 46,000 Mr, not significantly different from that of a molecule known to bear the Fya determinant [33].
  • Western blot analysis failed to detect CD14 in plasma from untreated CB6 (BALB/c x C57Bl6) mice, but showed markedly increased levels of CD14 in plasma from mice treated with lipopolysaccharide (LPS) [34].
  • Both phosphotyrosine and phosphoserine-specific monoclonal antibodies reacted with the 17-kD protein by Western blot, suggesting that the binding of SDH to these pharyngeal cells elicits a novel signaling pathway that ultimately leads to activation of histone H3-specific kinases [35].

Gene context of Blotting, Western


Analytical, diagnostic and therapeutic context of Blotting, Western

  • We determined the spatial localization of fibronectin in the embryo by immunofluorescence and the temporal program of its expression by biosynthesis studies and Western blotting techniques [41].
  • This analysis identified several potential shortcomings of the various methods used to evaluate HER-2/neu in these diseases (Southern, Northern, and Western blots, and immunohistochemistry) and provided information regarding considerations that should be addressed when studying a gene or gene product in human tissue [42].
  • Results for RT-PCR and Western blot analysis showed that COX I as well as an IL-1beta- and TNF-alpha-inducible COX II are expressed in HT-29 Cl.19A [43].
  • The levels of GC expression in progeny cells (primarily mature myelomonocytic) produced by the LTBMC were quantitatively analyzed by Northern blot, Western blot, and glucocerebrosidase enzyme assay [44].
  • Two noncompetitive monoclonal antibodies were used to examine by Western blotting the Factor VII cross-reactive material (CRM) in normal human plasma and three commercially available congenitally Factor VII-deficient plasmas, and to construct a facile "sandwich" immunoassay for plasma Factor VII [45].


  1. The BRCA1-interacting helicase BRIP1 is deficient in Fanconi anemia. Levran, O., Attwooll, C., Henry, R.T., Milton, K.L., Neveling, K., Rio, P., Batish, S.D., Kalb, R., Velleuer, E., Barral, S., Ott, J., Petrini, J., Schindler, D., Hanenberg, H., Auerbach, A.D. Nat. Genet. (2005) [Pubmed]
  2. Molecular cloning of a GTPase activating protein specific for the Krev-1 protein p21rap1. Rubinfeld, B., Munemitsu, S., Clark, R., Conroy, L., Watt, K., Crosier, W.J., McCormick, F., Polakis, P. Cell (1991) [Pubmed]
  3. Fatal familial insomnia, a prion disease with a mutation at codon 178 of the prion protein gene. Medori, R., Tritschler, H.J., LeBlanc, A., Villare, F., Manetto, V., Chen, H.Y., Xue, R., Leal, S., Montagna, P., Cortelli, P. N. Engl. J. Med. (1992) [Pubmed]
  4. Expression of the Qa-2k phenotype encoded by the Q5k gene on the surface of tumor cells derived from H-2k mice. Seo, N., Okazaki, T., Nakanishi-Ito, C., Tanino, T., Matsudaira, Y., Takahashi, T., Egawa, K. J. Exp. Med. (1992) [Pubmed]
  5. Infection of human thymocytes by Epstein-Barr virus. Watry, D., Hedrick, J.A., Siervo, S., Rhodes, G., Lamberti, J.J., Lambris, J.D., Tsoukas, C.D. J. Exp. Med. (1991) [Pubmed]
  6. Increased expression of the amyloid precursor beta-secretase in Alzheimer's disease. Holsinger, R.M., McLean, C.A., Beyreuther, K., Masters, C.L., Evin, G. Ann. Neurol. (2002) [Pubmed]
  7. Nitration of tau protein is linked to neurodegeneration in tauopathies. Horiguchi, T., Uryu, K., Giasson, B.I., Ischiropoulos, H., LightFoot, R., Bellmann, C., Richter-Landsberg, C., Lee, V.M., Trojanowski, J.Q. Am. J. Pathol. (2003) [Pubmed]
  8. No evidence for expanded polyglutamine sequences in bipolar disorder and schizophrenia. Jones, A.L., Middle, F., Guy, C., Spurlock, G., Cairns, N.J., McGuffin, P., Craddock, N., Owen, M., O'Donovan, M.C. Mol. Psychiatry (1997) [Pubmed]
  9. Studies of humoral immunity to preprohypocretin in human leukocyte antigen DQB1*0602-positive narcoleptic subjects with cataplexy. Black, J.L., Silber, M.H., Krahn, L.E., Avula, R.K., Walker, D.L., Pankratz, V.S., Fredrickson, P.A., Slocumb, N.L. Biol. Psychiatry (2005) [Pubmed]
  10. Alterations of protein kinase C isozyme and substrate proteins in mouse brain after electroconvulsive seizures. Chen, C.C. Brain Res. (1994) [Pubmed]
  11. The deoxyguanosine kinase gene is mutated in individuals with depleted hepatocerebral mitochondrial DNA. Mandel, H., Szargel, R., Labay, V., Elpeleg, O., Saada, A., Shalata, A., Anbinder, Y., Berkowitz, D., Hartman, C., Barak, M., Eriksson, S., Cohen, N. Nat. Genet. (2001) [Pubmed]
  12. Rickettsia africae, a tick-borne pathogen in travelers to sub-Saharan Africa. Raoult, D., Fournier, P.E., Fenollar, F., Jensenius, M., Prioe, T., de Pina, J.J., Caruso, G., Jones, N., Laferl, H., Rosenblatt, J.E., Marrie, T.J. N. Engl. J. Med. (2001) [Pubmed]
  13. ABCR expression in foveal cone photoreceptors and its role in Stargardt macular dystrophy. Molday, L.L., Rabin, A.R., Molday, R.S. Nat. Genet. (2000) [Pubmed]
  14. Apoptosis in the failing human heart. Olivetti, G., Abbi, R., Quaini, F., Kajstura, J., Cheng, W., Nitahara, J.A., Quaini, E., Di Loreto, C., Beltrami, C.A., Krajewski, S., Reed, J.C., Anversa, P. N. Engl. J. Med. (1997) [Pubmed]
  15. Urinary excretion of aquaporin-2 in patients with diabetes insipidus. Kanno, K., Sasaki, S., Hirata, Y., Ishikawa, S., Fushimi, K., Nakanishi, S., Bichet, D.G., Marumo, F. N. Engl. J. Med. (1995) [Pubmed]
  16. Carcinoma-specific Ulex europaeus agglutinin-I binding glycoproteins of human colorectal carcinoma and its relation to carcinoembryonic antigen. Matsushita, Y., Yonezawa, S., Nakamura, T., Shimizu, S., Ozawa, M., Muramatsu, T., Sato, E. J. Natl. Cancer Inst. (1985) [Pubmed]
  17. Antibodies to carbonic anhydrase in patients with immune cholangiopathies. Gordon, S.C., Quattrociocchi-Longe, T.M., Khan, B.A., Kodali, V.P., Chen, J., Silverman, A.L., Kiechle, F.L. Gastroenterology (1995) [Pubmed]
  18. Inability of enzyme immunoassays to discriminate between infections with herpes simplex virus types 1 and 2. Ashley, R., Cent, A., Maggs, V., Nahmias, A., Corey, L. Ann. Intern. Med. (1991) [Pubmed]
  19. Construction, expression, and immunogenicity of the Schistosoma mansoni P28 glutathione S-transferase as a genetic fusion to tetanus toxin fragment C in a live Aro attenuated vaccine strain of Salmonella. Khan, C.M., Villarreal-Ramos, B., Pierce, R.J., Riveau, G., Demarco de Hormaeche, R., McNeill, H., Ali, T., Fairweather, N., Chatfield, S., Capron, A. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  20. Heparin-associated thrombocytopenia: antibody binding specificity to platelet antigens. Lynch, D.M., Howe, S.E. Blood (1985) [Pubmed]
  21. Hierarchical phosphorylation of the translation inhibitor 4E-BP1. Gingras, A.C., Raught, B., Gygi, S.P., Niedzwiecka, A., Miron, M., Burley, S.K., Polakiewicz, R.D., Wyslouch-Cieszynska, A., Aebersold, R., Sonenberg, N. Genes Dev. (2001) [Pubmed]
  22. The expression and regulation of nitric oxide synthase in human osteoarthritis-affected chondrocytes: evidence for up-regulated neuronal nitric oxide synthase. Amin, A.R., Di Cesare, P.E., Vyas, P., Attur, M., Tzeng, E., Billiar, T.R., Stuchin, S.A., Abramson, S.B. J. Exp. Med. (1995) [Pubmed]
  23. Using cyclooxygenase-2 inhibitors as molecular platforms to develop a new class of apoptosis-inducing agents. Zhu, J., Song, X., Lin, H.P., Young, D.C., Yan, S., Marquez, V.E., Chen, C.S. J. Natl. Cancer Inst. (2002) [Pubmed]
  24. Effect of RNA silencing of polo-like kinase-1 (PLK1) on apoptosis and spindle formation in human cancer cells. Spänkuch-Schmitt, B., Bereiter-Hahn, J., Kaufmann, M., Strebhardt, K. J. Natl. Cancer Inst. (2002) [Pubmed]
  25. Expression of GTPase-deficient Ras inhibits vasopressin signaling in cultured cortical collecting duct cells. Wong, R., Heasley, L., Ao, L., Berl, T. J. Clin. Invest. (1995) [Pubmed]
  26. Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation. Smith, R.C., Dworkin, M.B., Dworkin-Rastl, E. Genes Dev. (1988) [Pubmed]
  27. Degradation of T cell receptor (TCR)-CD3-zeta complexes after antigenic stimulation. Valitutti, S., Müller, S., Salio, M., Lanzavecchia, A. J. Exp. Med. (1997) [Pubmed]
  28. The altered tumoricidal capacity of macrophages isolated from tumor-bearing mice is related to reduce expression of the inducible nitric oxide synthase gene. Dinapoli, M.R., Calderon, C.L., Lopez, D.M. J. Exp. Med. (1996) [Pubmed]
  29. Characterization of a functionally important and evolutionarily well-conserved epitope mapped to the short consensus repeats of E-selectin and L-selectin. Jutila, M.A., Watts, G., Walcheck, B., Kansas, G.S. J. Exp. Med. (1992) [Pubmed]
  30. HLA-DR associates with specific stress proteins and is retained in the endoplasmic reticulum in invariant chain negative cells. Schaiff, W.T., Hruska, K.A., McCourt, D.W., Green, M., Schwartz, B.D. J. Exp. Med. (1992) [Pubmed]
  31. The Duchenne muscular dystrophy gene product is localized in sarcolemma of human skeletal muscle. Zubrzycka-Gaarn, E.E., Bulman, D.E., Karpati, G., Burghes, A.H., Belfall, B., Klamut, H.J., Talbot, J., Hodges, R.S., Ray, P.N., Worton, R.G. Nature (1988) [Pubmed]
  32. Rapid latex agglutination assay using recombinant envelope polypeptide for the detection of antibody to the HIV. Quinn, T.C., Riggin, C.H., Kline, R.L., Francis, H., Mulanga, K., Sension, M.G., Fauci, A.S. JAMA (1988) [Pubmed]
  33. A new human Duffy blood group specificity defined by a murine monoclonal antibody. Immunogenetics and association with susceptibility to Plasmodium vivax. Nichols, M.E., Rubinstein, P., Barnwell, J., Rodriguez de Cordoba, S., Rosenfield, R.E. J. Exp. Med. (1987) [Pubmed]
  34. Murine CD14 gene expression in vivo: extramyeloid synthesis and regulation by lipopolysaccharide. Fearns, C., Kravchenko, V.V., Ulevitch, R.J., Loskutoff, D.J. J. Exp. Med. (1995) [Pubmed]
  35. Regulation of the phosphorylation of human pharyngeal cell proteins by group A streptococcal surface dehydrogenase: signal transduction between streptococci and pharyngeal cells. Pancholi, V., Fischetti, V.A. J. Exp. Med. (1997) [Pubmed]
  36. Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. Mylin, L.M., Bhat, J.P., Hopper, J.E. Genes Dev. (1989) [Pubmed]
  37. LYAR, a novel nucleolar protein with zinc finger DNA-binding motifs, is involved in cell growth regulation. Su, L., Hershberger, R.J., Weissman, I.L. Genes Dev. (1993) [Pubmed]
  38. Role of virion-associated glycosylphosphatidylinositol-linked proteins CD55 and CD59 in complement resistance of cell line-derived and primary isolates of HIV-1. Saifuddin, M., Parker, C.J., Peeples, M.E., Gorny, M.K., Zolla-Pazner, S., Ghassemi, M., Rooney, I.A., Atkinson, J.P., Spear, G.T. J. Exp. Med. (1995) [Pubmed]
  39. CD22 associates with protein tyrosine phosphatase 1C, Syk, and phospholipase C-gamma(1) upon B cell activation. Law, C.L., Sidorenko, S.P., Chandran, K.A., Zhao, Z., Shen, S.H., Fischer, E.H., Clark, E.A. J. Exp. Med. (1996) [Pubmed]
  40. Enhanced expression of heat shock protein 70 (hsp70) and heat shock factor 1 (HSF1) activation in rheumatoid arthritis synovial tissue. Differential regulation of hsp70 expression and hsf1 activation in synovial fibroblasts by proinflammatory cytokines, shear stress, and antiinflammatory drugs. Schett, G., Redlich, K., Xu, Q., Bizan, P., Gröger, M., Tohidast-Akrad, M., Kiener, H., Smolen, J., Steiner, G. J. Clin. Invest. (1998) [Pubmed]
  41. Temporal and spatial regulation of fibronectin in early Xenopus development. Lee, G., Hynes, R., Kirschner, M. Cell (1984) [Pubmed]
  42. Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Slamon, D.J., Godolphin, W., Jones, L.A., Holt, J.A., Wong, S.G., Keith, D.E., Levin, W.J., Stuart, S.G., Udove, J., Ullrich, A. Science (1989) [Pubmed]
  43. Identification of a human enterocyte lipoxin A4 receptor that is regulated by interleukin (IL)-13 and interferon gamma and inhibits tumor necrosis factor alpha-induced IL-8 release. Gronert, K., Gewirtz, A., Madara, J.L., Serhan, C.N. J. Exp. Med. (1998) [Pubmed]
  44. Retroviral-mediated transfer of the human glucocerebrosidase gene into cultured Gaucher bone marrow. Nolta, J.A., Yu, X.J., Bahner, I., Kohn, D.B. J. Clin. Invest. (1992) [Pubmed]
  45. Monoclonal anti-human factor VII antibodies. Detection in plasma of a second protein antigenically and genetically related to factor VII. Broze, G.J., Hickman, S., Miletich, J.P. J. Clin. Invest. (1985) [Pubmed]
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