Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Chemical Compound Review:

Gdpflrfamide (3S)-4-[(2S)-2-[[(1S)-1- [[(1S)-1-[[(1S)-1...

Synonyms: AC1MIZ2F, 110325-85-4, Gly-asp-pro-phe-leu-arg-phe-amide

Richmond, J.E. et al., Bulloch, A.G. et al., Salzet, M. et al., Worster, B.M. et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Gdpflrfamide

Bath application of FMRFamide, FLRFamide, or GDPFLRFamide at micromolar concentration to a buccal ganglia/salivary gland preparation completely suppressed spontaneous rhythmic activity [1].
Type 1 mRNA (exon II) encodes for the tetrapeptides (FLRF/FMRFamide), whereas Type 2 (exons III-V) encodes for the heptapeptides (SDPFLRFamide/GDPFLRFamide) [2].
Using enzyme-linked immunosorbent assays, a dot-immunobinding assay and a three-step reverse-phase HPLC separation, four Arg-Phe-amide (RFamide) peptides were purified from sex segmental ganglia extracts of the leech Erpobdella octoculata; FMRFamide, FM(O)RFamide, FLRFamide and GDPFLRFamide [3].
Bath application of the heptapeptide GDPFLRFamide (10(-5) mol l-1) reduced the calcium current by only 8% [4].

Analytical, diagnostic and therapeutic context of Gdpflrfamide

We report that, as determined by HPLC/RIA and mass spectrometry, Helisoma contains both FMRFamide and 2 of its analogs, FLRFamide and GDPFLRFamide [1].
The major FMRFamide-like peak in neurone VD4 appears to be FMRFamide itself, based on its high performance liquid chromatography (HPLC) elution time and immunoreactivity before and after oxidation, but small peaks are also present at the elution times of Phe-Leu-Arg-Phe-NH2 (FLRFamide) and Gly-Asp-Pro-Phe-Leu-Arg-Phe-NH2 (GDPFLRFamide) [4].

References

FMRFamide peptides in Helisoma: identification and physiological actions at a peripheral synapse. Bulloch, A.G., Price, D.A., Murphy, A.D., Lee, T.D., Bowes, H.N. J. Neurosci. (1988) [Pubmed]
Matrix-assisted laser desorption/ionization time of flight mass spectrometric analysis of the pattern of peptide expression in single neurons resulting from alternative mRNA splicing of the FMRFamide gene. Worster, B.M., Yeoman, M.S., Benjamin, P.R. Eur. J. Neurosci. (1998) [Pubmed]
FMRFamide-related peptides in the sex segmental ganglia of the Pharyngobdellid leech Erpobdella octoculata. Identification and involvement in the control of hydric balance. Salzet, M., Bulet, P., Wattez, C., Malecha, J. Eur. J. Biochem. (1994) [Pubmed]
Activation of a peptidergic synapse locally modulates postsynaptic calcium influx. Richmond, J.E., Funte, L.R., Smith, W.L., Price, D.A., Haydon, P.G. J. Exp. Biol. (1991) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

Editor

Share