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MeSH Review

Enzyme-Linked Immunosorbent Assay

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Disease relevance of Enzyme-Linked Immunosorbent Assay


Psychiatry related information on Enzyme-Linked Immunosorbent Assay

  • Amyloid-beta (Abeta) peptide levels are widely measured by enzyme-linked immunosorbent assay (ELISA) in Alzheimer's disease research [6].
  • Sera from mice treated with purified anti-idiotypic antibodies (anti-Id) were found to contain inhibitory activity in an ELISA specific for the 14-4-4S Id, whereas sera from control mice treated with heterologous normal Ig did not [7].
  • Specificity for PrP is confirmed by a sandwich enzyme-linked immunosorbent assay utilizing V5B2, which discriminates between CJD and normal samples without proteinase K treatment, and by immunoprecipitation from CJD brain homogenate [8].
  • This study shows that the current ELISA test for CSF tau is not sensitive and specific enough to distinguish early AD from other dementias and indicates that in the interpretation of CSF tau analysis as a diagnostic tool, the apoE genotype should also be taken into account [9].
  • In a study of Preventive Medicine and Education Program, fibrinogen (clotting method) and factor VIIag (ELISA), in addition to other metabolic parameters, life-style and familial history, were investigated in 451 students (313 females and 138 males, age 15-17 years) from two high schools of Florence [10].

High impact information on Enzyme-Linked Immunosorbent Assay


Chemical compound and disease context of Enzyme-Linked Immunosorbent Assay


Biological context of Enzyme-Linked Immunosorbent Assay


Anatomical context of Enzyme-Linked Immunosorbent Assay


Associations of Enzyme-Linked Immunosorbent Assay with chemical compounds

  • By the ELISA, MOMP-liposomes, but not plain liposomes lacking MOMP, avidly fix C3 [31].
  • To further confirm the presence of antihyaluronate antibodies, hyaluronidase-digested streptococcal hyaluronate was conjugated to biotin and used as an antigen in the ELISA [32].
  • Inhibition studies using an ELISA demonstrated that heparan sulfate was a major antigenic determinant [33].
  • Conditioned media samples prepared with cells from these mice, on day 2 after infection, produced 11-43 pg/million cells of IFN-gamma and 12-19 pg/million cells of TNF as evaluated by specific protein enzyme-linked immunosorbent assays [34].
  • Acid/ethanol extracts of human PMNs assayed by ELISA contained an average of 355 ng of TGF/beta 1 per 10(9) cells potentially available for secretion during degranulation of PMNs [35].

Gene context of Enzyme-Linked Immunosorbent Assay

  • MAIN OUTCOME MEASURES--Supernatants from trophoblast-activated peripheral blood mononuclear cells from all participants were tested for toxic effects on mouse embryos and by enzyme-linked immunosorbent assay (ELISA) for interferon gamma (IFN-gamma) [36].
  • Assembly of an extracellular fibronectin matrix by VHL-/- renal carcinoma cells, as determined by immunofluorescence and ELISA assays, was grossly defective compared with VHL+/+ renal carcinoma cells [37].
  • In addition, IL-2 was not detected (less than 50 pg/ml) in 16 joint samples using an ELISA [38].
  • Plasma samples (n = 454) were collected three times/week from all patients (n = 25) undergoing renal transplantation during a 9-month consecutive period, and assayed by ELISA and functional assays for TNF, PC, and free PS (FPS) [39].
  • Amyloid A fibrils in spleen homogenates were denatured by formic acid and AA protein was quantified by ELISA using anti-mouse apoSAA antibodies [40].

Analytical, diagnostic and therapeutic context of Enzyme-Linked Immunosorbent Assay


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  4. Respiratory infections with Pseudomonas aeruginosa in children with cystic fibrosis: early detection by serology and assessment of risk factors. West, S.E., Zeng, L., Lee, B.L., Kosorok, M.R., Laxova, A., Rock, M.J., Splaingard, M.J., Farrell, P.M. JAMA (2002) [Pubmed]
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  6. Amyloid-beta oligomers are inefficiently measured by enzyme-linked immunosorbent assay. Stenh, C., Englund, H., Lord, A., Johansson, A.S., Almeida, C.G., Gellerfors, P., Greengard, P., Gouras, G.K., Lannfelt, L., Nilsson, L.N. Ann. Neurol. (2005) [Pubmed]
  7. Idiotypes of anti-Ia antibodies. II. Effects of in vivo treatment with xenogeneic anti-idiotype. Epstein, S.L., Masakowski, V.R., Sharrow, S.O., Bluestone, J.A., Ozato, K., Sachs, D.H. J. Immunol. (1982) [Pubmed]
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  10. Fibrinogen and factor VIIag in healthy adolescents: the Floren-teen (Florence teenager) Study. Prisco, D., Fedi, S., Brunelli, T., Cellai, A.P., Hagi, M.I., Gianni, R., Santoro, E., Cappelletti, C., Pepe, G., Gensini, G.F., Abbate, R. Thromb. Haemost. (1996) [Pubmed]
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  12. Prevalence of hepatitis C virus RNA in organ donors positive for hepatitis C antibody and in the recipients of their organs. Pereira, B.J., Milford, E.L., Kirkman, R.L., Quan, S., Sayre, K.R., Johnson, P.J., Wilber, J.C., Levey, A.S. N. Engl. J. Med. (1992) [Pubmed]
  13. Antibodies to HTLV-III in Swiss patients with AIDS and pre-AIDS and in groups at risk for AIDS. Schüpbach, J., Haller, O., Vogt, M., Lüthy, R., Joller, H., Oelz, O., Popovic, M., Sarngadharan, M.G., Gallo, R.C. N. Engl. J. Med. (1985) [Pubmed]
  14. Postcoital detection of a male-specific semen protein. Application to the investigation of rape. Graves, H.C., Sensabaugh, G.F., Blake, E.T. N. Engl. J. Med. (1985) [Pubmed]
  15. Fas ligand in human serum. Tanaka, M., Suda, T., Haze, K., Nakamura, N., Sato, K., Kimura, F., Motoyoshi, K., Mizuki, M., Tagawa, S., Ohga, S., Hatake, K., Drummond, A.H., Nagata, S. Nat. Med. (1996) [Pubmed]
  16. Safety and immunogenicity of a serogroups A/C Neisseria meningitidis oligosaccharide-protein conjugate vaccine in young children. A randomized controlled trial. Lieberman, J.M., Chiu, S.S., Wong, V.K., Partidge, S., Chang, S.J., Chiu, C.Y., Gheesling, L.L., Carlone, G.M., Ward, J.I. JAMA (1996) [Pubmed]
  17. Fv structure of monoclonal antibody II-481 against herpes simplex virus Fc gamma-binding glycoprotein gE contains immunodominant complementarity determining region epitopes that react with human immunoglobulin M rheumatoid factors. Johansson, P.J., Malone, C., Swietnicki, W., Dunn, B.M., Williams, R.C. J. Exp. Med. (1994) [Pubmed]
  18. Tissue transglutaminase autoantibody enzyme-linked immunosorbent assay in detecting celiac disease. Sulkanen, S., Halttunen, T., Laurila, K., Kolho, K.L., Korponay-Szabó, I.R., Sarnesto, A., Savilahti, E., Collin, P., Mäki, M. Gastroenterology (1998) [Pubmed]
  19. Presence of autoantibodies to interleukin-8 or neutrophil-activating peptide-2 in patients with heparin-associated thrombocytopenia. Amiral, J., Marfaing-Koka, A., Wolf, M., Alessi, M.C., Tardy, B., Boyer-Neumann, C., Vissac, A.M., Fressinaud, E., Poncz, M., Meyer, D. Blood (1996) [Pubmed]
  20. Immunological specificity and mechanism of action of IgG lupus anticoagulants. Pengo, V., Thiagarajan, P., Shapiro, S.S., Heine, M.J. Blood (1987) [Pubmed]
  21. Suppression of anti-DNA antibody synthesis in vitro by a cross-reactive antiidiotypic antibody. Epstein, A., Greenberg, M., Diamond, B., Grayzel, A.I. J. Clin. Invest. (1987) [Pubmed]
  22. Complex formation of platelet thrombospondin with plasminogen. Modulation of activation by tissue activator. Silverstein, R.L., Leung, L.L., Harpel, P.C., Nachman, R.L. J. Clin. Invest. (1984) [Pubmed]
  23. Modified low density lipoprotein and its constituents augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells. Khan, B.V., Parthasarathy, S.S., Alexander, R.W., Medford, R.M. J. Clin. Invest. (1995) [Pubmed]
  24. Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen. Chan, E.K., Hamel, J.C., Buyon, J.P., Tan, E.M. J. Clin. Invest. (1991) [Pubmed]
  25. Complement activation by Cremophor EL as a possible contributor to hypersensitivity to paclitaxel: an in vitro study. Szebeni, J., Muggia, F.M., Alving, C.R. J. Natl. Cancer Inst. (1998) [Pubmed]
  26. 9-O-Acetylation of sialomucins: a novel marker of murine CD4 T cells that is regulated during maturation and activation. Krishna, M., Varki, A. J. Exp. Med. (1997) [Pubmed]
  27. Cytokines and arachidonic metabolites produced during human immunodeficiency virus (HIV)-infected macrophage-astroglia interactions: implications for the neuropathogenesis of HIV disease. Genis, P., Jett, M., Bernton, E.W., Boyle, T., Gelbard, H.A., Dzenko, K., Keane, R.W., Resnick, L., Mizrachi, Y., Volsky, D.J. J. Exp. Med. (1992) [Pubmed]
  28. A critical role for the programmed death ligand 1 in fetomaternal tolerance. Guleria, I., Khosroshahi, A., Ansari, M.J., Habicht, A., Azuma, M., Yagita, H., Noelle, R.J., Coyle, A., Mellor, A.L., Khoury, S.J., Sayegh, M.H. J. Exp. Med. (2005) [Pubmed]
  29. Resolution of cutaneous leishmaniasis: interleukin 12 initiates a protective T helper type 1 immune response. Sypek, J.P., Chung, C.L., Mayor, S.E., Subramanyam, J.M., Goldman, S.J., Sieburth, D.S., Wolf, S.F., Schaub, R.G. J. Exp. Med. (1993) [Pubmed]
  30. Mouse monoclonal antibody to embryonic antigen: development, cross-reactivity with rodent and human tumors, and preliminary polypeptide characterization. Payne, W.J., Coggin, J.H. J. Natl. Cancer Inst. (1985) [Pubmed]
  31. Complement component C3 fixes selectively to the major outer membrane protein (MOMP) of Legionella pneumophila and mediates phagocytosis of liposome-MOMP complexes by human monocytes. Bellinger-Kawahara, C., Horwitz, M.A. J. Exp. Med. (1990) [Pubmed]
  32. Induction of antibodies to hyaluronic acid by immunization of rabbits with encapsulated streptococci. Fillit, H.M., McCarty, M., Blake, M. J. Exp. Med. (1986) [Pubmed]
  33. Sera from patients with poststreptococcal glomerulonephritis contain antibodies to glomerular heparan sulfate proteoglycan. Fillit, H., Damle, S.P., Gregory, J.D., Volin, C., Poon-King, T., Zabriskie, J. J. Exp. Med. (1985) [Pubmed]
  34. Requirement for natural killer cell-produced interferon gamma in defense against murine cytomegalovirus infection and enhancement of this defense pathway by interleukin 12 administration. Orange, J.S., Wang, B., Terhorst, C., Biron, C.A. J. Exp. Med. (1995) [Pubmed]
  35. Transforming growth factor beta 1 (TGF-beta 1) induced neutrophil recruitment to synovial tissues: implications for TGF-beta-driven synovial inflammation and hyperplasia. Fava, R.A., Olsen, N.J., Postlethwaite, A.E., Broadley, K.N., Davidson, J.M., Nanney, L.B., Lucas, C., Townes, A.S. J. Exp. Med. (1991) [Pubmed]
  36. T-helper 1-type immunity to trophoblast in women with recurrent spontaneous abortion. Hill, J.A., Polgar, K., Anderson, D.J. JAMA (1995) [Pubmed]
  37. The von Hippel-Lindau tumor suppressor protein is required for proper assembly of an extracellular fibronectin matrix. Ohh, M., Yauch, R.L., Lonergan, K.M., Whaley, J.M., Stemmer-Rachamimov, A.O., Louis, D.N., Gavin, B.J., Kley, N., Kaelin, W.G., Iliopoulos, O. Mol. Cell (1998) [Pubmed]
  38. Cytokines in chronic inflammatory arthritis. I. Failure to detect T cell lymphokines (interleukin 2 and interleukin 3) and presence of macrophage colony-stimulating factor (CSF-1) and a novel mast cell growth factor in rheumatoid synovitis. Firestein, G.S., Xu, W.D., Townsend, K., Broide, D., Alvaro-Gracia, J., Glasebrook, A., Zvaifler, N.J. J. Exp. Med. (1988) [Pubmed]
  39. Tumor necrosis factor production during human renal allograft rejection is associated with depression of plasma protein C and free protein S levels and decreased intragraft thrombomodulin expression. Tsuchida, A., Salem, H., Thomson, N., Hancock, W.W. J. Exp. Med. (1992) [Pubmed]
  40. Linkage of protection against amyloid fibril formation in the mouse to a single, autosomal dominant gene. Gonnerman, W.A., Elliott-Bryant, R., Carreras, I., Sipe, J.D., Cathcart, E.S. J. Exp. Med. (1995) [Pubmed]
  41. Transmission of hepatitis C virus by organ transplantation. Pereira, B.J., Milford, E.L., Kirkman, R.L., Levey, A.S. N. Engl. J. Med. (1991) [Pubmed]
  42. AIDS serology testing in low- and high-risk groups. Carlson, J.R., Bryant, M.L., Hinrichs, S.H., Yamamoto, J.K., Levy, N.B., Yee, J., Higgins, J., Levine, A.M., Holland, P., Gardner, M.B. JAMA (1985) [Pubmed]
  43. Human autoantibody-reactive epitopes of SS-B/La are highly conserved in comparison with epitopes recognized by murine monoclonal antibodies. Chan, E.K., Tan, E.M. J. Exp. Med. (1987) [Pubmed]
  44. High level IL-12 production by murine dendritic cells: upregulation via MHC class II and CD40 molecules and downregulation by IL-4 and IL-10. Koch, F., Stanzl, U., Jennewein, P., Janke, K., Heufler, C., Kämpgen, E., Romani, N., Schuler, G. J. Exp. Med. (1996) [Pubmed]
  45. Long-lasting, specific immunologic unresponsiveness associated with cryptococcal meningitis. Henderson, D.K., Bennett, J.E., Huber, M.A. J. Clin. Invest. (1982) [Pubmed]
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