Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Chemical Compound Review:

AC1Q5HJP 3,4,6-trihydroxy-2,5-dioxo- hexanoic acid

Synonyms: AR-1J1877, AC1L18EM, 159171-61-6, hexo-2,5-diulosonic acid, 3,4,6-trihydroxy-2,5-dioxohexanoic acid

Miller, J.V. et al., Carper, D.A. et al., Verma, V. et al.

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Disease relevance of 25-DIKETO-D-GLUCONATE

Purification and characterization of 2,5-diketo-D-gluconate reductase from Corynebacterium sp [1].

High impact information on 25-DIKETO-D-GLUCONATE

The enzyme is active over a broad pH range (pH 5-8) for the reduction of 2,5-diketo-D-gluconate; a sharp optimum at pH 9.2 is observed for the oxidation of 2-keto-L-gulonate [1].
At 25 degrees C, pH 6.4, the turnover number is 500 min-1, and the apparent Km values for 2,5-diketo-D-gluconate and NADPH are 26 mM and 10 microM, respectively [1].
By comparing protein sequences, we have found that the structural relatedness (41% to 57%) among the vertebrate proteins, aldose reductase, aldehyde reductase, prostaglandin F synthase and the frog lens protein rho-crystallin can now be extended to prokaryotes by the inclusion of Corynebacterium 2,5-diketo-D-gluconate reductase [2].
A fast spheroplast formation procedure in some 2,5-diketo-D-gluconate- and 2-keto-L-gulonate- producing bacteria [3].

References

Purification and characterization of 2,5-diketo-D-gluconate reductase from Corynebacterium sp. Miller, J.V., Estell, D.A., Lazarus, R.A. J. Biol. Chem. (1987) [Pubmed]
A superfamily of NADPH-dependent reductases in eukaryotes and prokaryotes. Carper, D.A., Wistow, G., Nishimura, C., Graham, C., Watanabe, K., Fujii, Y., Hayashi, H., Hayaishi, O. Exp. Eye Res. (1989) [Pubmed]
A fast spheroplast formation procedure in some 2,5-diketo-D-gluconate- and 2-keto-L-gulonate- producing bacteria. Verma, V., Qazi, G.N., Parshad, R., Chopra, C.L. BioTechniques (1989) [Pubmed]

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