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Gene Review: 

nifB  -  nitrogen fixation protein

Nostoc sp. PCC 7120

 
 
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Disease relevance of nifB

  • Comparison of the nifB 5'-flanking sequence with the nifH 5'-flanking sequence did not reveal any consensus base pairs that would define a nif promoter for Anabaena [1].
 

High impact information on nifB

  • It is located just upstream of the nifHDK operon and consists of four genes in the order nifB, fdxN, nifS, and nifU [1].
  • The operon contains two instances of 7-base pair directly repeated sequences: seven copies of the repeated sequence are found between the nifB and fdxN genes and six copies are found between the nifS and nifU genes [1].
  • The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined [2].
 

Biological context of nifB

  • The promoter for the nifB operon was located by primer extension [1].

References

  1. Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120. The nifB-fdxN-nifS-nifU operon. Mulligan, M.E., Haselkorn, R. J. Biol. Chem. (1989) [Pubmed]
  2. Bacterial-type ferredoxin genes in the nitrogen fixation regions of the cyanobacterium Anabaena sp. strain PCC 7120 and Rhizobium meliloti. Mulligan, M.E., Buikema, W.J., Haselkorn, R. J. Bacteriol. (1988) [Pubmed]
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