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Gene Review:

Atu4292 - chloramphenicol resistance protein

Agrobacterium fabrum str. C58

Shaw, W.V. et al., Vlasák, J. et al., Ratet, P. et al., Tennigkeit, J. et al.

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Disease relevance of Atu4292

Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product [1].

High impact information on Atu4292

The directly determined primary structure is identical with that predicted by the DNA sequence analysis of the chloramphenicol resistance transponson Tn9 known to specify a type I variant of chloramphenicol acetyltransferase [2].
To provide a more solid basis for studying the evolution and spread of CAT within prokaryotes we chose to determine the complete amino acid sequence of a type I variant of CAT, the variant known to be associated with most F-like plasmids conferring chloramphenicol resistance [2].
Different selectable markers (chloramphenicol resistance; gentamycin resistance) were introduced to allow selection for mini-Mu insertions in different replicons and bacterial strains [3].
The chloramphenicol resistance gene from pSa was introduced into T-DNA of pTi T37 of Agrobacterium tumefaciens by cointegration with intermediary plasmid based on pBR322 [4].
Agrocin sensitivity of tranconjugants together with chloramphenicol resistance was shown to be a sufficient and simple criterion of transfer of modified Ti plasmids [4].

References

Nucleotide sequence analysis of a chloramphenicol-resistance determinant from Agrobacterium tumefaciens and identification of its gene product. Tennigkeit, J., Matzura, H. Gene (1991) [Pubmed]
Primary structure of a chloramphenicol acetyltransferase specified by R plasmids. Shaw, W.V., Packman, L.C., Burleigh, B.D., Dell, A., Morris, H.R., Hartley, B.S. Nature (1979) [Pubmed]
Mini-Mulac transposons with broad-host-range origins of conjugal transfer and replication designed for gene regulation studies in Rhizobiaceae. Ratet, P., Schell, J., de Bruijn, F.J. Gene (1988) [Pubmed]
Modification of T-DNA of nopaline Ti plasmid by intermediary vector and utilization of agrocin 84 sensitivity as simple criterion of conjugation transfer of modified Ti plasmid. Vlasák, J., Hrouda, M., Bísková, R., Ondrej, M. Folia Microbiol. (Praha) (1986) [Pubmed]

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