Disease relevance of Agrocin

• Agrobacterium radiobacter K84, used worldwide to biocontrol crown gall disease caused by Agrobacterium tumefaciens, produces an antiagrobacterial compound called agrocin 84 [1].
• Substituents at N6 and C-5' control selective uptake and toxicity of the adenine-nucleotide bacteriocin, agrocin 84, in Agrobacteria [2].
• The agrocin plasmid could not be introduced into restrictionless Escherichia coli or Pseudomonas aeruginosa recipients by conjugation or transformation [3].

High impact information on Agrocin

• Bases of biocontrol: sequence predicts synthesis and mode of action of agrocin 84, the Trojan horse antibiotic that controls crown gall [1].
• We report the nucleotide sequence of pAgK84, a 44.42-kb plasmid coding for production of this disubstituted adenine nucleotide antibiotic. pAgK84 encodes 36 ORFs, 17 of which (agn) code for synthesis of or immunity to agrocin 84 [1].
• The accF mutant was not susceptible to agrocin 84 although it took up the antibiotic [4].
• Characterization of the acc operon from the nopaline-type Ti plasmid pTiC58, which encodes utilization of agrocinopines A and B and susceptibility to agrocin 84 [4].
• The acc locus from the Ti plasmid pTiC58 confers utilization of and chemotaxis toward agrocinopines A and B (A+B), as well as susceptibility to a highly specific antiagrobacterial antibiotic, agrocin 84 [4].

Chemical compound and disease context of Agrocin

• Genetic analysis of the agrocinopine catabolic region of Agrobacterium tumefaciens Ti plasmid pTiC58, which encodes genes required for opine and agrocin 84 transport [5].

Biological context of Agrocin

• Plasmid pAgK84-encoded immunity to agrocin 84 was located to two separate regions of the plasmid [6].
• The data are consistent with a single operon encoding catabolism of agrocinopines A and B and conferring sensitivity to agrocin 84 [7].
• The loss of plasmids from virulent transconjugants during growth at 37 degrees C indicated that virulence genes reside on pAr15834b, whereas agrocin 84 sensitivity genes reside on pAr15834a [8].
• Growth rates and phenotypes of streptomycin- or rifampin-resistant K84 were similar to those of the parental K84, except the rifampin-resistant mutant produced less agrocin 84 as determined by bioassay [9].
• Equilibrium dialysis studies also indicate that the N6 substituent is 'recognised' by a binding protein involved in the uptake of agrocin 84 into sensitive strains [2].

Anatomical context of Agrocin

• Biochemical studies on cytoplasmic membranes of agrocin sensitive and resistant strains of Agrobacterium tumefaciens [10].

Gene context of Agrocin

• In strains also harboring pAtK84b, the opine catabolic plasmid of Agrobacterium radiobacter K84, induction of the agrocinopine A catabolic locus of this plasmid had no such effect on agrocin 84 production [6].
• Correlation of deletions with concomitant loss of virulence and agrocin 84 sensitivity identified the megaplasmid pAtAB2/73d as the virulence element in strain AB2/73 [11].
• Agrocin sensitivity of tranconjugants together with chloramphenicol resistance was shown to be a sufficient and simple criterion of transfer of modified Ti plasmids [12].
• Modification of T-DNA of nopaline Ti plasmid by intermediary vector and utilization of agrocin 84 sensitivity as simple criterion of conjugation transfer of modified Ti plasmid [12].

References