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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

tme  -  malic enzyme

Sinorhizobium meliloti 1021

 
 
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High impact information on tme

  • The tme gene was placed under the control of the dme promoter, and despite elevated levels of TME within bacteroids, no symbiotic nitrogen fixation occurred in dme mutant strains [1].
  • We constructed strains carrying insertions within the tme gene region and showed that the NADP+ -dependent malic enzyme activity peak was absent when extracts from these strains were eluted from a DEAE-cellulose chromatography column [2].
  • Here, we report the cloning and localization of the tme gene (encoding the triphosphopyridine nucleotide [NADP+]-dependent malic enzyme) to a 3.7-kb region [2].
 

Biological context of tme

  • The functions of DME and the NADP+ -dependent malic enzyme are discussed in light of the above results and the growth phenotypes of various tme and dme mutants [2].
 

Associations of tme with chemical compounds

  • We found that NADP+ -dependent malic enzyme activity was not required for N2 fixation, as tme mutants induced N2-fixing root nodules on alfalfa [2].

References

  1. Malic Enzyme Cofactor and Domain Requirements for Symbiotic N2 Fixation by Sinorhizobium meliloti. Mitsch, M.J., Cowie, A., Finan, T.M. J. Bacteriol. (2007) [Pubmed]
  2. NADP+ -dependent malic enzyme of Rhizobium meliloti. Driscoll, B.T., Finan, T.M. J. Bacteriol. (1996) [Pubmed]
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