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Chemical Compound Review

NADP     [(2R,3R,4R,5R)-5- [[[[(2R,3S,4R,5R)-5-(5...

Synonyms: beta-TPN, beta-NADP, TPN-ox, Codehydrase II, TPN+, ...
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Disease relevance of triphosphopyridine nucleotide


Psychiatry related information on triphosphopyridine nucleotide

  • When longer reaction times were used with NADPH and NADP(+), a mixture of 7 alpha-OH-DHEA, 7 beta-OH-DHEA, and 7-oxo-DHEA was produced (19,14, and 35 nmol/180 min/2 ml, respectively; 62% conversion) [6].

High impact information on triphosphopyridine nucleotide

  • From the insoluble pellet fraction of cultured skin fibroblast homogenates, released glucose was measured enzymically using hexokinase coupled with the glucose-6-phosphate dehydrogenase (G6PD) and nicotinamide adenine dinucleotide phosphate (NADP) system [7].
  • In contrast, although sickle RBCs had a significant increase in the total NADP content compared with normal RBCs (P less than .00005), sickle RBCs had no significant alteration in the NADPH/[NADP+ + NADPH] ratio [8].
  • Parasite G6PD exhibited much higher affinity (low Km) to G6P and nicotinamide-adenine dinucleotide phosphate (NADP) than did human G6PD [9].
  • Kinetic evidence for the dimerization of the triphosphopyridine nucleotide-dependent isocitrate dehydrogenase from pig heart [10].
  • Nicotinic acid adenine dinucleotide phosphate (NAADP), a molecule derived from beta-NADP, has been shown to trigger Ca2+ release from intracellular stores of invertebrate eggs and mammalian cell microsomes [11].

Chemical compound and disease context of triphosphopyridine nucleotide


Biological context of triphosphopyridine nucleotide


Anatomical context of triphosphopyridine nucleotide

  • We found that NAADP, but not beta-NADP, activates a specific microsomal calcium release system in mesangial cells isolated from rat kidney; NAADP was without effect in renal tubular epithelial cells [19].
  • Addition of 1 mM Ca or of 1 nM IGF I to the medium (0.3 mM Ca) of a rat bone-derived cell line, PyMS, stimulated not only DNA synthesis but also sodium-dependent (Nad) phosphate (Pi) uptake, the latter, within 2 h [20].
  • Cytochemical evidence for the existence of a Golgi-associated phosphatase activity that hydrolyzes nicotinamide adenine dinucleotide phosphate (NADP) at acid pH in rat incisor ameloblasts was obtained by incubating sections from glutaraldehyde-fixed teeth in a medium containing NADP as substrate and lead ions as capture agent [21].
  • As well, no deposits of reaction product were seen within the Golgi saccules of ameloblasts incubated at pH 5.0 with nictoinamide adenine dinucleotide (NAD) as the substrate or that were incubated at pH 7.2 or pH 9.0 with NADP as the substrate [21].
  • It was eliminated by dialysis of the cytosol and reduced by omission of nicotinamide adenine dinucleotide phosphate (NADP) from the reaction mixture [22].

Associations of triphosphopyridine nucleotide with other chemical compounds

  • We have found that the (1)H spectrum of NADP(+) in the presence of the R67 DHFR changes as a function of time [23].
  • NAD, NADP, and cholesterol showed no luminescence signal possibly due to the very low absorption coefficient at 355 nm [24].
  • In this method, the enzyme label is used to catalyse the dephosphorylation of nicotinamide adenine dinucleotide phosphate (NADP+); the NAD+ so formed then catalytically activates an NAD+-specific redox cycle, yielding an intensely coloured formazan dye [25].
  • Biochemical characteristics including enzyme activity, electrophoretic mobility, Km for glucose-6-phosphate (G6P) and nicotinamide adenine dinucleotide phosphate (NADP), heat stability and pH optimum of all the common and deficient variants were consistent with the reported characteristics of these variants [26].
  • We have now investigated the reverse G-6-PD reaction, namely, the oxidation of reduced nicotinamide-adenine dinucleotide phosphate (NADPH) by 6-phosphoglucono-delta-lactone to form glucose-6-phosphate and nicotinamide-adenine dinucleotide phosphate (NADP) [27].

Gene context of triphosphopyridine nucleotide

  • This we accomplished using glucose-6-phosphate dehydrogenase and a surplus amount of NADP, followed by elimination of reduced NADP by acidification of the reaction mixture [28].
  • Similarly, lower detection limit (4.0x10(-5) M) for the measurement of G6P was achieved using glucose-6-phosphate dehydrogenase (G6PDH) in the presence of beta-NADP(+) [29].
  • Analysis of the more titration-sensitive DHFR amide resonances as a function of added NADP(+) gave a K(D) of 131 +/- 50 microM, consistent with previous determinations using other methodology [23].
  • Oxidation-reduction midpoint potentials (E(m)) have been measured for the thioredoxin-dependent, reductive activation of sorghum nicotinamide adenine dinucleotide phosphate- (NADP-) dependent malate dehydrogenase (MDH) in the wild-type enzyme and in a number of site-specific mutants [30].
  • The catalytic mechanism proposed for ferredoxin-NADP(+) reductase (FNR) is initiated by reduction of its flavin adenine dinucleotide (FAD) cofactor by the obligatory one-electron carriers ferredoxin (Fd) or flavodoxin (Fld) in the presence of oxidized nicotinamide adenine dinucleotide phosphate (NADP(+)) [31].

Analytical, diagnostic and therapeutic context of triphosphopyridine nucleotide

  • The amount of antibody bound ALP conjugate was determined by its activity in dephosphorylating p-nitrophenyl phosphate in EIA and nicotinamide adenine dinucleotide phosphate (NADP+) in ELISA [32].
  • Relative Glut-1 levels were determined by Western immunoblot analysis using human anti-Glut-1 rabbit polyclonal antibody, and hexokinase activity was measured in the same samples by an enzymatic assay monitoring the reduction in the oxidized form of nicotinamide adenine dinucleotide phosphate (NADP+) (in nmol NADP+ reduced/min per mg protein) [33].
  • The products arising from one-electron electrochemical reduction of the coenzyme nicotinamide adenine dinucleotide phosphate (NADP+) have been studied by HPLC chromatography and 1H-NMR spectroscopy [34].


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  8. Decreased erythrocyte nicotinamide adenine dinucleotide redox potential and abnormal pyridine nucleotide content in sickle cell disease. Zerez, C.R., Lachant, N.A., Lee, S.J., Tanaka, K.R. Blood (1988) [Pubmed]
  9. Glucose-6-phosphate dehydrogenase of malaria parasite Plasmodium falciparum. Yoshida, A., Roth, E.F. Blood (1987) [Pubmed]
  10. Kinetic evidence for the dimerization of the triphosphopyridine nucleotide-dependent isocitrate dehydrogenase from pig heart. Kelly, J.H., Plaut, G.W. J. Biol. Chem. (1981) [Pubmed]
  11. Nicotinic acid adenine dinucleotide phosphate: a new Ca2+ releasing agent in kidney. Cheng, J., Yusufi, A.N., Thompson, M.A., Chini, E.N., Grande, J.P. J. Am. Soc. Nephrol. (2001) [Pubmed]
  12. pH-tuneable binding of 2'-phospho-ADP-ribose to ketopantoate reductase: a structural and calorimetric study. Ciulli, A., Lobley, C.M., Tuck, K.L., Smith, A.G., Blundell, T.L., Abell, C. Acta Crystallogr. D Biol. Crystallogr. (2007) [Pubmed]
  13. The role of 6-phosphogluconate dehydrogenase in Rhizobium. Mulongoy, K., Elkan, G.H. Can. J. Microbiol. (1977) [Pubmed]
  14. Coenzyme binding by triphosphopyridine nucleotide dependent isocitrate dehydrogenase from beef liver. Equilibrium and kinetics studies. Carlier, M.F., Pantaloni, D. Biochemistry (1976) [Pubmed]
  15. Cloning and characterization of a novel all-trans retinol short-chain dehydrogenase/reductase from the RPE. Wu, B.X., Chen, Y., Chen, Y., Fan, J., Rohrer, B., Crouch, R.K., Ma, J.X. Invest. Ophthalmol. Vis. Sci. (2002) [Pubmed]
  16. Morphology-associated expression nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase in Mucorracemosus. Peters, J., Sypherd, P.S. J. Bacteriol. (1979) [Pubmed]
  17. Oxygenic photoreduction of methyl viologen and nicotinamide adenine dinucleotide phosphate without the involvement of photosystem I during plastid development. Daniell, H., Anbudurai, P.R., Periyannan, S., Renganathan, M., Bhardwaj, R., Kulandaivelu, G., Gnanam, A. Biochem. Biophys. Res. Commun. (1985) [Pubmed]
  18. Purification and properties of glucose 6-phosphate dehydrogenase from Aspergillus aculeatus. Ibraheem, O., Adewale, I.O., Afolayan, A. J. Biochem. Mol. Biol. (2005) [Pubmed]
  19. Nicotinic acid-adenine dinucleotide phosphate (NAADP) elicits specific microsomal Ca2+ release from mammalian cells. Yusufi, A.N., Cheng, J., Thompson, M.A., Chini, E.N., Grande, J.P. Biochem. J. (2001) [Pubmed]
  20. Calcium and insulin-like growth factor I stimulation of sodium-dependent phosphate transport and proliferation of cultured rat osteoblasts. Schmid, C., Keller, C., Schläpfer, I., Veldman, C., Zapf, J. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  21. Ultrastructural localization of nicotinamide adenine dinucleotide phosphatase (NADPase) activity to the intermediate saccules of the Golgi apparatus in rat incisor ameloblasts. Smith, C.E. J. Histochem. Cytochem. (1980) [Pubmed]
  22. Enhancing activity of rat tissue extracts for induction of lambda prophage by L-azaserine. Suit, J.L., Miranda-da Cruz, B., Sito, L., Rogers, A.E. Environmental mutagenesis. (1984) [Pubmed]
  23. NMR studies of the interaction of a type II dihydrofolate reductase with pyridine nucleotides reveal unexpected phosphatase and reductase activity. Pitcher, W.H., DeRose, E.F., Mueller, G.A., Howell, E.E., London, R.E. Biochemistry (2003) [Pubmed]
  24. Singlet oxygen generation by UVA light exposure of endogenous photosensitizers. Baier, J., Maisch, T., Maier, M., Engel, E., Landthaler, M., Bäumler, W. Biophys. J. (2006) [Pubmed]
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  29. Voltammetric biosensors for the determination of formate and glucose-6-phosphate based on the measurement of dehydrogenase-generated NADH and NADPH. Hung Tzang, C., Yuan, R., Yang, M. Biosensors & bioelectronics. (2001) [Pubmed]
  30. Oxidation-reduction properties of the regulatory disulfides of sorghum chloroplast nicotinamide adenine dinucleotide phosphate-malate dehydrogenase. Hirasawa, M., Ruelland, E., Schepens, I., Issakidis-Bourguet, E., Miginiac-Maslow, M., Knaff, D.B. Biochemistry (2000) [Pubmed]
  31. Role of the C-terminal tyrosine of ferredoxin-nicotinamide adenine dinucleotide phosphate reductase in the electron transfer processes with its protein partners ferredoxin and flavodoxin. Nogués, I., Tejero, J., Hurley, J.K., Paladini, D., Frago, S., Tollin, G., Mayhew, S.G., Gómez-Moreno, C., Ceccarelli, E.A., Carrillo, N., Medina, M. Biochemistry (2004) [Pubmed]
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  33. Variability in glucose transporter-1 levels and hexokinase activity in human melanoma. Wachsberger, P.R., Gressen, E.L., Bhala, A., Bobyock, S.B., Storck, C., Coss, R.A., Berd, D., Leeper, D.B. Melanoma Res. (2002) [Pubmed]
  34. 1H-NMR study and structure determination of 4,4- and 4,6-dimers from electrochemical reduction of NADP+. Ragg, E., Scaglioni, L., Mondelli, R., Carelli, V., Carelli, I., Casini, A., Finazzi-Agrò, A., Liberatore, F., Tortorella, S. Biochim. Biophys. Acta (1991) [Pubmed]
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