Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

denA - site-specific endonuclease II (EndoII)...

Enterobacteria phage T4

Yee, J.K. et al., Miller, E.S. et al., Mattson, T. et al., Carlson, K. et al., Mattson, T. et al.

Miller, Shih, Chang, Ballard, Carlson, Kosturko, Nyström,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of denA

The 136 codon (408 bp) denA gene encoding endonuclease II (Endoll) of bacteriophage T4 was unambiguously identified through sequencing and subsequent cloning [1].
An E. coli B Tab strain, EM121, was isolated that restricts T4 denA (DNA endonuclease II) mutants at 37 degrees C and above, but is permissive for wild-type T4 at all temperatures examined [2].

High impact information on denA

However, neither plasmid or chromosomal degradation can be detected in denA mutant infections by the method of DNA--DNA hybridization on nitrocellulose filters [3].
DNA fragments from a BglII digestion of cytosine-containing DNA from a T4 dCTPase- denA denB(rIIH23B) alc mutant were hybridized with full-length chromosomal strands of bacteriophage T2, and the heteroduplexes were examined by electron microscopy [4].
Bacteriophage T4 DNA containing cytosine has been obtained from cells infected with phage mutant in genes 42, 56, denA and denB [5].

References

Sequence-specific cleavage by bacteriophage T4 endonuclease II in vitro. Carlson, K., Kosturko, L.D., Nyström, A.C. Mol. Microbiol. (1999) [Pubmed]
An E. coli B mutation, rpoB5081, that prevents growth of phage T4 strains defective in host DNA degradation. Miller, E.S., Shih, G.C., Chang, S.K., Ballard, D.N. FEMS Microbiol. Lett. (1997) [Pubmed]
Fate of cloned bacteriophage T4 DNA after phage T4 infection of clone-bearing cells. Mattson, T., Van Houwe, G., Bolle, A., Epstein, R. J. Mol. Biol. (1983) [Pubmed]
Alignment of a restriction map with the genetic map of bacteriophage T4. Yee, J.K., Marsh, R.C. J. Virol. (1981) [Pubmed]
Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes. Mattson, T., Van Houwe, G., Bolle, A., Selzer, G., Epstein, R. Mol. Gen. Genet. (1977) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.