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Gene Review: 

tRNA-Cys  -  tRNA

Kazachstania servazzii

 
 
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High impact information on tRNA-Cys

  • The transcripts of the genes for tRNAThrACN and tRNACys (the tRNA genes immediately downstream from the 21S rRNA gene) have been analyzed; the possibility that these species represent primary transcripts is considered, and potential sites for initiation of transcription of the clustered tRNA genes are discussed [1].
  • The levels of the tRNAs varied even more markedly, ranging from 4200 copies/cell for the tRNAPhe to 240 copies/cell for the tRNACys after growth in derepressive conditions and from 800 copies/cell for the tRNAfMet to 30 copies/cell for the tRNACys of glucose repressed yeast [2].
  • The strengths of the promoters of the genes were ordered: tRNAMetf greater than tRNAPhe greater than 14 S rRNA greater than 21 S rRNA greater than tRNAGlu greater than Oli-1 much greater than tRNACys [3].
  • 5. Chromatography of tRNA Cys on Bio-Gel P100 polyacrylamide beads afforded two components one of which was far less efficient than the other in accepting cysteine [4].

References

  1. Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts. Palleschi, C., Francisci, S., Zennaro, E., Frontali, L. EMBO J. (1984) [Pubmed]
  2. Steady state analysis of mitochondrial RNA after growth of yeast Saccharomyces cerevisiae under catabolite repression and derepression. Mueller, D.M., Getz, G.S. J. Biol. Chem. (1986) [Pubmed]
  3. Transcriptional regulation of the mitochondrial genome of yeast Saccharomyces cerevisiae. Mueller, D.M., Getz, G.S. J. Biol. Chem. (1986) [Pubmed]
  4. The extraction and purification of a cysteine transfer ribonucleic acid from baker's yeast. Holness, N.J., Atfield, G. Biochem. J. (1976) [Pubmed]
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