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Gene Review:

tufA - elongation factor 1-alpha

Chlamydomonas reinhardtii

Kawazoe, R. et al., Vernon, D. et al., Silk, G.W. et al., Silk, G.W. et al.

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High impact information on tufA

Nucleotide sequence of the chloroplast gene for the 4 kD K polypeptide of photosystem II (psbK) and the psbK-tufA intergenic region of Chlamydomonas reinhardtii [1].
Two plastid genes--rrn16, encoding the plastid small-subunit rRNA, and tufA, encoding elongation factor Tu--retain their functions in protein synthesis after the loss of photosynthesis in two nonphotosynthetic Polytoma clades but show a substantially accelerated rate of base substitution in the P. uvella clade [2].
This effect was shown to be mainly transcriptional, and not restricted to tufA, as transcription of other chloroplast-encoded genes were cycloheximide-sensitive, although not all equally (psbA showed no more than 40% inhibition) [3].
Cycloheximide, an inhibitor of cytoplasmic translation, induced a rapid reduction of 70-80% in levels of mRNA for the chloroplast elongation factor Tu (tufA) in asynchronously growing Chlamydomonas [3].

Other interactions of tufA

When etiolated cc-125 y-1 cells were illuminated for 5 h, a 1710 bp tufA mRNA accumulated up to 5-fold while the psbA, rbcL, and 16S rRNA transcripts accumulated less than 1.5-fold [4].

References

Nucleotide sequence of the chloroplast gene for the 4 kD K polypeptide of photosystem II (psbK) and the psbK-tufA intergenic region of Chlamydomonas reinhardtii. Silk, G.W., Dela Cruz, F., Wu, M. Nucleic Acids Res. (1990) [Pubmed]
Accelerated evolution of functional plastid rRNA and elongation factor genes due to reduced protein synthetic load after the loss of photosynthesis in the chlorophyte alga Polytoma. Vernon, D., Gutell, R.R., Cannone, J.J., Rumpf, R.W., Birky, C.W. Mol. Biol. Evol. (2001) [Pubmed]
Requirement for cytoplasmic protein synthesis during circadian peaks of transcription of chloroplast-encoded genes in Chlamydomonas. Kawazoe, R., Hwang, S., Herrin, D.L. Plant Mol. Biol. (2000) [Pubmed]
Posttranscriptional accumulation of chloroplast tufA (elongation factor gene) mRNA during chloroplast development in Chlamydomonas reinhardtii. Silk, G.W., Wu, M. Plant Mol. Biol. (1993) [Pubmed]

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