Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Gene Review:

gyrA - DNA gyrase subunit A

Bacillus anthracis str. Ames Ancestor

Hurtle, W. et al., Harrell, L.J. et al., Hurtle, W. et al., Price, L.B. et al.

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Disease relevance of gyrA

Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe [1].
The 16S-23S ISR was analyzed by this method with 42 strains of B. anthracis, 36 strains of Bacillus cereus, and 12 strains of Bacillus thuringiensis; the gyrA gene was analyzed by this method with 33 strains of B. anthracis, 27 strains of B. cereus, and 9 strains of B. thuringiensis [2].

High impact information on gyrA

Ciprofloxacin MICs were 0.5 micro g/ml for first-step mutants, which had one of two gyrA quinolone resistance-determining region (QRDR) mutations [3].
After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains [1].
We evaluated the abilities of pulsed-field gel electrophoresis (PFGE) and sequences of intergenic spacer regions (ISRs) between two highly conserved genes, 16S-23S rDNA and gyrB-gyrA ISRs, to detect variation in strains of Bacillus anthracis as well as two closely related species, B. cereus ATCC 14579 and B. mycoides ATCC 6462 [4].

Analytical, diagnostic and therapeutic context of gyrA

Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene [2].

References

Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe. Hurtle, W., Bode, E., Kulesh, D.A., Kaplan, R.S., Garrison, J., Bridge, D., House, M., Frye, M.S., Loveless, B., Norwood, D. J. Clin. Microbiol. (2004) [Pubmed]
Use of denaturing high-performance liquid chromatography to identify Bacillus anthracis by analysis of the 16S-23S rRNA interspacer region and gyrA gene. Hurtle, W., Bode, E., Kaplan, R.S., Garrison, J., Kearney, B., Shoemaker, D., Henchal, E., Norwood, D. J. Clin. Microbiol. (2003) [Pubmed]
In vitro selection and characterization of Bacillus anthracis mutants with high-level resistance to ciprofloxacin. Price, L.B., Vogler, A., Pearson, T., Busch, J.D., Schupp, J.M., Keim, P. Antimicrob. Agents Chemother. (2003) [Pubmed]
Genetic variability of Bacillus anthracis and related species. Harrell, L.J., Andersen, G.L., Wilson, K.H. J. Clin. Microbiol. (1995) [Pubmed]

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