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Gene Review:

GSTK1 - glutathione S-transferase kappa 1

Homo sapiens

Synonyms: GST, GST 13-13, GST class-kappa, GST13, GST13-13, ...

Morel, F. et al., Li, J. et al., Robinson, A. et al.

Robinson, Huttley, Booth, Board, Morel, Rauch, Petit, Piton, Theret, Coles, Guillouzo,

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Disease relevance of GSTK1

Recombinant hGSTK1-1 was expressed in Escherichia coli as a homodimer (subunit molecular mass approximately 25.5 kDa) [1].

High impact information on GSTK1

The C terminus of hGSTK1 was essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal [2].
The peroxisomal localization was confirmed by transfection of HepG2 cells with a plasmid coding a green fluorescent protein fused inframe to the N terminus of hGSTK1 [2].
The 5'-flanking region lacks TATA or CCAAT boxes, but there is an initiator element overlapping the transcription start site. hGSTK1 amino acid sequence showed homology to bacterial 2-hydroxychromene-2-carboxylate isomerase, an enzyme involved in naphthalene degradation pathway. hGSTK1 mRNA was expressed in all of the organs examined [2].
Each subunit of the dimeric hGSTK contains a thioredoxin (TRX)-like domain and a helical domain [3].
The overall structure of hGSTK is similar to the recently reported structure of kappa class GST from rat mitochondrion [3].

References

Modelling and bioinformatics studies of the human Kappa-class glutathione transferase predict a novel third glutathione transferase family with similarity to prokaryotic 2-hydroxychromene-2-carboxylate isomerases. Robinson, A., Huttley, G.A., Booth, H.S., Board, P.G. Biochem. J. (2004) [Pubmed]
Gene and protein characterization of the human glutathione S-transferase kappa and evidence for a peroxisomal localization. Morel, F., Rauch, C., Petit, E., Piton, A., Theret, N., Coles, B., Guillouzo, A. J. Biol. Chem. (2004) [Pubmed]
Thioredoxin-like domain of human kappa class glutathione transferase reveals sequence homology and structure similarity to the theta class enzyme. Li, J., Xia, Z., Ding, J. Protein Sci. (2005) [Pubmed]

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GSTK1	Bos taurus
gstk-1	Caenorhabditis elegans
GSTK1	Canis lupus familiaris
si:ch211-198d13.2	Danio rerio
LOC100537097	Danio rerio
zgc:136861	Danio rerio
LOC101885925	Danio rerio
gstk1	Danio rerio
GSTK1	Gallus gallus
GSTK1	Macaca mulatta
Gstk1	Mus musculus
GSTK1	Pan troglodytes
Gstk1	Rattus norvegicus
LOC101730645	Xenopus (Silurana) tropicalis
gstk1	Xenopus (Silurana) tropicalis

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