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Gene Review

DIABLO  -  diablo, IAP-binding mitochondrial protein

Bos taurus

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Disease relevance of DIABLO

  • Increased incubation temperature (42 degrees C) and treatment with half of the prescribed amount of cefixime-tellurite by BAM for SMAC agar in enrichment step were the most effective in selectively growing E. coli O157:H7 [1].

High impact information on DIABLO

  • Indole positives colonies were plated on SMAC agar with 20 microg/ml nalidixic acid (SMACnal) [2].
  • MacConkey medium containing sorbitol, tellurite and cefixime (TC-SMAC) permitted the growth of VT+ E. coli O157 and Shigella sonnei but partially or completely inhibited the growth of 67% of other strains of E. coli and all or most strains of other sorbitol-non-fermenting species tested [3].

Analytical, diagnostic and therapeutic context of DIABLO

  • METHODS AND RESULTS: Minced beef and bovine hide were inoculated with varying concentrations (log(10) 1.58-2.58 CFU g(-1) and log(10) 2.42-4.49 CFU 100 cm(2) respectively) of E. coli O157:H7 and recovered using a direct plate method or an enrichment/immunomagnetic separation (IMS) method and then plated onto SMAC or SMAC-CT in both cases [4].


  1. Evaluation of increased incubation temperature and cefixime-tellurite treatment for the isolation of Escherichia coli O157:H7 from minced beef. Dogan, H.B., Kuleasan, H., Cakir, I., Halkman, A.K. Int. J. Food Microbiol. (2003) [Pubmed]
  2. Effect of pooling bovine fecal samples on the sensitivity of detection of E. coli O157:H7. Sanderson, M.W., Sargeant, J.M., Nagaraja, T.G. Vet. Microbiol. (2005) [Pubmed]
  3. Use of tellurite for the selection of verocytotoxigenic Escherichia coli O157. Zadik, P.M., Chapman, P.A., Siddons, C.A. J. Med. Microbiol. (1993) [Pubmed]
  4. Detection and recovery rates achieved using direct plate and enrichment/immunomagnetic separation methods for Escherichia coli O157:H7 in minced beef and on bovine hide. O'Brien, S.B., Duffy, G., Daly, D., Sheridan, J.J., Blair, I.S., McDowell, D.A. Lett. Appl. Microbiol. (2005) [Pubmed]
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