Disease relevance of SUP6

• Although the SUP6 delta fragment is contained within a 750-bp restriction fragment, isolated from Saccharomyces cerevisiae, and contains no encoded CCA, the primary transcript, which originates from an E. coli promoter, approx. 1000 bp upstream, is processed to a mature, functional transfer RNA [1].

High impact information on SUP6

• Precise deletion of the intervening sequence of a yeast tRNATyr ochre suppressor gene (SUP6) significantly reduced its suppressor activity relative to that of the unaltered gene [2].
• This ochre pre-tRNA(Ile) mutant has the central anticodon uridine modified to psi 35 as is the case for S.cerevisiae SUP6 tyrosine-inserting ochre suppressor tRNA [3].
• We have replaced the 14 nucleotide long intervening sequence of the Saccharomyces cerevisiae SUP6 (ochre) tRNA(Tyr) gene by the 52 nucleotide long second intron of the S. cerevisiae MATa1 gene [4].
• Tagetitoxin inhibition of in vitro transcription of the yeast SUP4 and SUP6 tRNA(Tyr) genes demonstrates template dependence and indicates that inhibition may occur after UMP incorporation [5].
• Spontaneous secondary mutations of the ochre suppressor SUP6 were selected in a haploid strain of Saccharomyces cerevisiae [6].

Chemical compound and disease context of SUP6

• The SUP6 delta encoded tRNA, although coding for tRNATyr in yeast, is not charged solely with tyrosine in E. coli [1].

Biological context of SUP6

• Gene conversion and intragenic recombination at the SUP6 locus and the surrounding region in Saccharomyces cerevisiae [6].
• Fifteen open reading frames (ORFs) greater than 100 amino acids and one tRNA-Tyr gene (SUP6) were detected [7].

References