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Gene Review:

PRP28 - Prp28p

Saccharomyces cerevisiae S288c

Synonyms: Helicase CA8, Pre-mRNA-splicing ATP-dependent RNA helicase PRP28, YD8419.10C, YDR243C

Strauss, E.J. et al., Chen, J.Y. et al., Brenner, T.J. et al., Staley, J.P. et al.

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High impact information on PRP28

In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5 [1].
Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable [1].
These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing [1].
The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28) [1].
Here, we show that the function of an otherwise essential DEAD box protein, Prp28p, can be bypassed by mutations that alter either the protein U1-C or the U1 small nuclear RNA [2].

Biological context of PRP28

Using genetics, we identified the DExD/H box protein Prp28p as a potential mediator of the switch [3].

Anatomical context of PRP28

The allele snu114-60, which truncates the C terminus, was synthetically lethal with factors required for activation of the spliceosome, including the DExD/H-box ATPases BRR2 and PRP28 [4].

Analytical, diagnostic and therapeutic context of PRP28

The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family of proteins that are thought to function as ATP-dependent RNA helicases [1].

References

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family. Strauss, E.J., Guthrie, C. Genes Dev. (1991) [Pubmed]
Specific alterations of U1-C protein or U1 small nuclear RNA can eliminate the requirement of Prp28p, an essential DEAD box splicing factor. Chen, J.Y., Stands, L., Staley, J.P., Jackups, R.R., Latus, L.J., Chang, T.H. Mol. Cell (2001) [Pubmed]
An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p. Staley, J.P., Guthrie, C. Mol. Cell (1999) [Pubmed]
Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Brenner, T.J., Guthrie, C. Genetics (2005) [Pubmed]

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AGOS_AFR452C	Ashbya gossypii ATCC 10895
KLLA0C15433g	Kluyveromyces lactis NRRL Y-1140

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