High impact information on DIG1

• DIG2 is pheromone-inducible, whereas DIG1 is constitutively expressed [1].
• Ste12 also binds specifically to GST-Dig1 in the absence of any other yeast protein [1].
• High-level expression of Dig1 suppresses invasive growth and also causes cells to appear more resistant to pheromone-imposed cell cycle arrest [1].
• Furthermore, disruption of dig1, but not dig2, causes elevated transcriptional activation by a LexA-Ste12p(216-688) fusion [2].
• The phenotype of rst1 rst2 cells required Ste12 function, but did not require the function of upstream kinases [3].

Biological context of DIG1

• Expression of DIG2, but not DIG1, from a GAL promoter inhibits transcriptional activation by an Ste12p DBD-VP16 fusion [2].
• Homozygous deletion of both RST1 and RST2 (rst-) causes a/alpha diploid cells constitutively to express a-specific genes and mate preferentially as a-cells [4].
• The encoded protein has a single DNA binding homeodomain but lacks both a C2H2 zinc finger DNA binding domain and an apparent Dig1/Dig2 regulatory motif [5].

Analytical, diagnostic and therapeutic context of DIG1

• Using the two-hybrid method, both Dig1 and Dig2 also interact with the other MAP kinase of the pheromone response pathway, Fus3 [1].

References