Disease relevance of PRO3

• The deduced amino acid sequence showed 25% identity with that of the Escherichia coli delta 1-pyrroline-5-carboxylate reductase, an enzyme of the pathway for the biosynthesis of proline [1].

High impact information on PRO3

• The product of this enzyme was identified as P5C by the fact that it was converted to proline by chemical reduction with NaBH4 or by enzymatic reduction with NADH in the presence of purified yeast P5C reductase [2].
• Isolation and sequence analysis of the cDNA encoding delta 1-pyrroline-5-carboxylate reductase from Zalerion arboricola [3].
• The presence of the PRO3 gene on a high-copy-number plasmid in S. cerevisiae caused a 20-fold overproduction of delta 1-pyrroline-5-carboxylate reductase [4].
• When the P5C was derived from ornithine, it was first converted to proline by the enzyme P5C reductase [5].
• The recessive mutations defined four complementation groups, named ore1, ore2, ore3 and ore4 [1].

Biological context of PRO3

• ore2, a mutation affecting proline biosynthesis in the yeast Saccharomyces cerevisiae, leads to a cdc phenotype [1].

Associations of PRO3 with chemical compounds

• In addition, the chromosomal location of the gene and its restriction map were compatible with those previously reported for the PRO3 gene which encodes the S. cerevisiae delta 1-pyrroline-5-carboxylate reductase [1].
• A pro3 mutant has been shown by enzyme assay to be deficient in delta 1-pyrroline-5-carboxylate reductase which converts pyrroline-5-carboxylate to proline [6].

References