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Gene Review:

ksgA - dimethyladenosine transferase

Escherichia coli O157:H7 str. Sakai

Heus, H.A. et al., Van Buul, C.P. et al., Lafontaine, D. et al., Suvorov, A.N. et al., Formenoy, L.J. et al.

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Disease relevance of ksgA

The methylation reaction was studied in vitro using ribosomes from a kasugamycin resistant mutant (ksgA) of Escherichia coli and purified methyl-transferase [1].
The 3'-terminal colicin fragments of 16S ribosomal RNA were isolated from Bacillus stearothermophilus and from its kasugamycin-resistant (ksgA) derivative lacking N6-dimethylation of the two adjacent adenosines in a hairpin loop [2].

High impact information on ksgA

Disruption of ksgA confers resistance to the aminoglycoside antibiotic kasugamycin without significantly impairing viability [3].
The conserved U.G base pair does not of itself appear to play a major role in ksgA methylase recognition [4].
It was also shown by in vitro experiments that ribosomes from erm ksgA cells have become sensitive to kasugamycin [5].

References

Partial methylation of two adjacent adenosines in ribosomes from Euglena gracilis chloroplasts suggests evolutionary loss of an intermediate stage in the methyl-transfer reaction. Van Buul, C.P., Hamersma, M., Visser, W., Van Knippenberg, P.H. Nucleic Acids Res. (1984) [Pubmed]
Conformational and thermodynamic effects of naturally occurring base methylations in a ribosomal RNA hairpin of Bacillus stearothermophilus. Heus, H.A., Formenoy, L.J., Van Knippenberg, P.H. Eur. J. Biochem. (1990) [Pubmed]
The DIM1 gene responsible for the conserved m6(2)Am6(2)A dimethylation in the 3'-terminal loop of 18 S rRNA is essential in yeast. Lafontaine, D., Delcour, J., Glasser, A.L., Desgrès, J., Vandenhaute, J. J. Mol. Biol. (1994) [Pubmed]
Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3' terminal hairpin. Formenoy, L.J., Cunningham, P.R., Nurse, K., Pleij, C.W., Ofengand, J. Biochimie (1994) [Pubmed]
Increased kasugamycin sensitivity in Escherichia coli caused by the presence of an inducible erythromycin resistance (erm) gene of Streptococcus pyogenes. Suvorov, A.N., van Gemen, B., van Knippenberg, P.H. Mol. Gen. Genet. (1988) [Pubmed]

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