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Gene Review:

glnD - uridylyltransferase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0165, JW0162

Rhee, S.G. et al., Tøndervik, A. et al., Garcia, E. et al.

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Disease relevance of glnD

The purification was facilitated by the use of an E. coli strain which carries multiple copies of a ColE1-hybrid plasmid containing the glnD gene that encodes uridylyltransferase and which overproduces its synthesis by 25-fold [1].
In order to study the converter enzymes which exist in extremely low concentration, the glnD and glnE genes were cloned into a plasmid vector carrying the strong, regulatable lambda phage promoter [2].

High impact information on glnD

The recombinant DNA technology also enabled us to examine the transcriptional regulation of the glnD and glnE genes [2].
We report that chromosomal mini-Tn10 insertions situated after nucleotide number 997 or 1075 of glnD partially suppressed the osmosensitive phenotype of DeltaotsBA or otsA::Tn10 mutations (defective osmoregulatory trehalose synthesis) [3].

References

Cascade control of Escherichia coli glutamine synthetase. Purification and properties of PII uridylyltransferase and uridylyl-removing enzyme. Garcia, E., Rhee, S.G. J. Biol. Chem. (1983) [Pubmed]
The role of adenylyltransferase and uridylyltransferase in the regulation of glutamine synthetase in Escherichia coli. Rhee, S.G., Park, S.C., Koo, J.H. Curr. Top. Cell. Regul. (1985) [Pubmed]
Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli. Tøndervik, A., Torgersen, H.R., Botnmark, H.K., Strøm, A.R. J. Bacteriol. (2006) [Pubmed]

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