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Gene Review:

glnK - nitrogen assimilation regulatory protein...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0444, JW0440, ybaI

van Heeswijk, W.C. et al., Tøndervik, A. et al., Ehlers, C. et al.

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Disease relevance of glnK

We cloned and overexpressed glnK and found that the expressed protein had almost the same molecular weight as PII, reacted with polyclonal PII antibody, and was 67% identical in terms of amino acid sequence with Escherichia coli PII [1].

High impact information on glnK

We describe a new operon, glnK amtB, which encodes a homologue of PII and a putative ammonia transporter [1].
Because of a NRI and a sigma N (sigma 54) RNA polymerase-binding consensus sequence upstream from the glnK gene, this suggests that glnK is regulated through the NRI/NRII two-component regulatory system [1].
GlnD catalyzes these reactions in response to high or low levels of cytoplasmic glutamine, respectively, and indirectly directs the expression of nitrogen-regulated genes, e.g., the glnK-amtB operon [2].
This was in contrast to the much-studied glnD99::Tn10 mutation, which carries its insertion in the 3' end of the gene, causes a complete repression of glnKp-lacZ expression under all growth conditions, and also confers leaky glutamine auxotrophy [2].
Because M. mazei GlnK1 was able to complement growth of an E. coli glnK mutant with arginine as the sole nitrogen source, it is likely that uridylylation is not required for its regulatory function [3].

References

An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli. van Heeswijk, W.C., Hoving, S., Molenaar, D., Stegeman, B., Kahn, D., Westerhoff, H.V. Mol. Microbiol. (1996) [Pubmed]
Transposon mutations in the 5' end of glnD, the gene for a nitrogen regulatory sensor, that suppress the osmosensitive phenotype caused by otsBA lesions in Escherichia coli. Tøndervik, A., Torgersen, H.R., Botnmark, H.K., Strøm, A.R. J. Bacteriol. (2006) [Pubmed]
Characterization of GlnK1 from Methanosarcina mazei strain Gö1: complementation of an Escherichia coli glnK mutant strain by GlnK1. Ehlers, C., Grabbe, R., Veit, K., Schmitz, R.A. J. Bacteriol. (2002) [Pubmed]

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