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Gene Review

amyA  -  cytoplasmic alpha-amylase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1926, JW1912, yedC
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Disease relevance of amyA

  • The high level of DNA similarity between the E. coli and S. typhimurium sequences that exists in flagellar region IIIa and in amyA continues initially, with three genes of unknown function; in E. coli, there may be a fourth gene [1].

High impact information on amyA

  • The amyA gene represented a monocistronic operon [2].
  • It was located in the amy gene region of the chromosome downstream of the pullulanase-encoding amyB gene and upstream of amyDC, encoding membrane components of an ABC transport system, and the alpha-amylase gene amyA [2].
  • Three plasmid vectors have been created using either the glucoamylase A (amyA), pyruvate decarboxylase (pdcA), or phosphoglycerate kinase (pgk1) promoters to drive expression of heterologous proteins [3].
  • The amylase gene (amyA) consisted of an open reading frame of 1,845 bp encoding a protein of 613 amino acids with a calculated mol. wt of 69,543 [4].
  • The gene amyA consists of 1764 base pairs and was predicted to encode a 587-amino acid protein encompassing a 31-amino acid signal peptide [5].


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