Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

ssrA - miscRNA

Escherichia coli O157:H7 str. EDL933

Kirby, J.E. et al., Garza-S??nchez, F. et al., Karaolis, D.K. et al., McGinness, K.E. et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of ssrA

In E. coli, the adaptor SspB tethers ssrA-tagged substrates to the ClpXP protease, causing a modest increase in their rate of degradation [1].
The PAI is 39.5 kb in size, has low %G+C (35%), contains putative integrase and transposase genes, is flanked by att sites, and inserts near a 10Sa RNA gene (ssrA), suggesting it may be of bacteriophage origin [2].
Excision and loss of the cryptic prophage apparently allow expression of the Alp protease by inactivating a small stable RNA (10Sa RNA) encoded by the ssrA gene [3].

High impact information on ssrA

Although A-site-cleaved mRNAs were not detected, tmRNA-mediated ssrA tagging after SecM glycine 165 was observed [4].
Indeed, compensatory overexpression of tRNA(2)(Pro) readily inhibits ssrA tagging after glycine 165, but has no effect on the duration of SecM ribosome arrest [4].
Inactivation of ssrA by insertional mutagenesis is sufficient to allow expression of the suppressing Alp protease, even in the presence of the cryptic prophage [3].

References

Engineering controllable protein degradation. McGinness, K.E., Baker, T.A., Sauer, R.T. Mol. Cell (2006) [Pubmed]
A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Karaolis, D.K., Johnson, J.A., Bailey, C.C., Boedeker, E.C., Kaper, J.B., Reeves, P.R. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
Excision of a P4-like cryptic prophage leads to Alp protease expression in Escherichia coli. Kirby, J.E., Trempy, J.E., Gottesman, S. J. Bacteriol. (1994) [Pubmed]
Prolyl-tRNAPro in the A-site of SecM-arrested Ribosomes Inhibits the Recruitment of Transfer-messenger RNA. Garza-S??nchez, F., Janssen, B.D., Hayes, C.S. J. Biol. Chem. (2006) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.