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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Absorption of trigonelline from the small intestine of the specific pathogen-free (SPF) and germ-free (GF) rats in vivo.

After the direct injection of trigonelline (Tg) to the upper end of the duodenum in SPF and GF rats, the remaining Tg in the contents of small intestine was determined after fractionation by ion-exchange and Nucher column chromatography, and by using a high performance thin-layer chromatography (HPTLC). When Tg was injected to the upper end of the duodenum of SPF rats, the remaining Tg in the sac markedly decreased with time. The results obtained from the experiment with GF rats almost correspond to that with SPF rats. It was therefore clear that Tg is not destroyed by the small intestinal microflora and a greater part of Tg is absorbed from the small intestine.[1]


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