The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Editor

Share

Personal info

View

Links

About

Terms

 

 
 

P(i) inhibits the SR Ca(2+) pump and stimulates pump-mediated Ca(2+) leak in rabbit cardiac myocytes.

Measurements of sarcoplasmic reticulum (SR) Ca(2+) uptake were made from aliquots of dissociated permeabilized ventricular myocytes using fura 2. Equilibration with 10 mM oxalate ensured a reproducible exponential decline of [Ca(2+)] from 600 nM to a steady state of 100-200 nM after addition of Ca(2+). In the presence of 5 microM ruthenium red, which blocks the ryanodine receptor, the time course of the decline of [Ca(2+)] can be modeled by a Ca(2+)-dependent uptake process and a fixed Ca(2+) leak. Partial inhibition of the Ca(2+) pump with 1 microM cyclopiazonic acid or 50 nM thapsigargin reduced the time constant for Ca(2+) uptake but did not affect the SR Ca(2+) leak. Addition of 10 mM inorganic phosphate (P(i)) decreased the rate of Ca(2+) accumulation by the SR and increased the Ca(2+) leak rate. This effect was reversed on addition of 10 mM phosphocreatine. 10 mM P(i) had no effect on Ca(2+) leak from the SR after complete inhibition of the Ca(2+) pump. In conclusion, P(i) decreases the Ca(2+) uptake capacity of cardiac SR via a decrease in pump rate and an increase in Ca(2+) pump-dependent Ca(2+) leak.[1]

References

  1. P(i) inhibits the SR Ca(2+) pump and stimulates pump-mediated Ca(2+) leak in rabbit cardiac myocytes. Smith, G.L., Duncan, A.M., Neary, P., Bruce, L., Burton, F.L. Am. J. Physiol. Heart Circ. Physiol. (2000) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
 
WikiGenes - Universities