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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Demonstration of Cl- requirement for inhibition of vacuolar acidification by cycloprodigiosin in situ.

Applying vacuole-perfusion and plasma membrane permeabilization techniques to internodal cells of Chara, we analyzed the requirement of Cl- for the action of cycloprodigiosin (cPrG) to inhibit vacuole acidification in situ. By combining the two techniques, the Cl- concentration on both sides of the tonoplast could be controlled. In permeabilized cell fragments lacking Cl- in the vacuole, the inhibitory effect of cPrG on vacuole acidification was cancelled. On the other hand, Cl- in the cytoplasm was not needed for the cPrG action. These results supported the function of cPrG as a H+/Cl- symporter. Requirement of Cl- for the cPrG action was also demonstrated in vacuole-perfused living cells. This is the first report on the mechanism of cPrG action in situ.[1]

References

  1. Demonstration of Cl- requirement for inhibition of vacuolar acidification by cycloprodigiosin in situ. Nakayasu, T., Kawauchi, K., Hirata, H., Shimmen, T. Plant Cell Physiol. (2000) [Pubmed]
 
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