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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Phosphate uptake and release by Acinetobacter johnsonii in continuous culture and coupling of phosphate release to heavy metal accumulation.

A strain of polyphosphate-synthesizing, phosphate-releasing Acinetobacter johnsonii was isolated from a wastewater treatment plant operating enhanced biological phosphate removal (EBPR) and was used to remove La(3+) from solution via precipitation of cell-bound LaPO(4). The effect of repeated aerobic-anaerobic cycles on the carbon and phosphate metabolism of the organism was studied in attempts to promote increased phosphate flux using a three-stage, continuous bioreactor comprising aerobic, anaerobic and settling vessels. The bioreactor was operated in two modes: In flow-through mode, cells were grown aerobically with acetate as the sole carbon source, promoting excess phosphate uptake (up to 5.0 mmol/l=3.0 mmol/g protein). Cells were diluted into the anaerobic vessel where phosphate was released (up to 1.0 mmol/l=0.3 mmol/g protein), and thence to waste. The system was initially operated to steady state in flow-through mode, then switched to recycle mode. Here the anaerobic vessel output passed to a settling vessel from which settled cells were returned to the aerobic vessel. Carbon source (acetate) was supplied only to the anaerobic vessel; increased anaerobic acetate uptake was observed during recycle, which was sustained when the system was returned to flow-through mode and was related to increased cellular lipid inclusions by flow cytometry and electron microscopy. These phenomena may represent adaptation of cells to aerobic-anaerobic cycling with aerobic carbon/energy limitation. Addition of La(3+) to the anaerobic vessel during recycle mode promoted removal of 95% of the La(3+) from a 0.1 to 0.3 mM (14-42 ppm) solution at the expense of biogenic phosphate.[1]

References

  1. Phosphate uptake and release by Acinetobacter johnsonii in continuous culture and coupling of phosphate release to heavy metal accumulation. Boswell, C.D., Dick, R.E., Eccles, H., Macaskie, L.E. J. Ind. Microbiol. Biotechnol. (2001) [Pubmed]
 
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