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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA.

The recovery of recombinant influenza A virus entirely from cDNA was recently described (9, 19). We adapted the technique for engineering influenza B virus and generated a mutant bearing an amino acid change E116G in the viral neuraminidase which was resistant in vitro to the neuraminidase inhibitor zanamivir. The method also facilitates rapid isolation of single-gene reassortants suitable as vaccine seeds and will aid further investigations of unique features of influenza B virus.[1]

References

  1. A reverse genetics approach for recovery of recombinant influenza B viruses entirely from cDNA. Jackson, D., Cadman, A., Zurcher, T., Barclay, W.S. J. Virol. (2002) [Pubmed]
 
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