Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Lee, H.S. et al.

Transcriptional regulation of Xbr-1a/Xvent-2 homeobox gene: analysis of its promoter region.

Xvent homeobox proteins are induced by BMP-4 signaling and have been known to mediate many BMP-4 activities as key downstream transcriptional factors. In order to investigate the regulatory mode of Xvent transcription, we isolated genomic DNA of the Xbr-1a/Xvent-2 containing the promoter region responsive to BMP-4 signaling. The cis-acting elements located within the Xbr-1a/Xvent-2 promoter and the regulation modes by BMP-4 signaling were analyzed by serial deletion and site-directed mutagenesis experiments. The upstream -235bp of the promoter retained the full transcriptional activity and BMP-4-response when compared with the longest promoter construct. Further analysis indicated that two separated 15bp regions contained a strong positive element and BMP-4-response element. Site-directed mutagenesis of those regions suggests that those two regions cooperate for the promoter activity and BMP-4-response. Moreover, we found that the transcription factors, Oaz and PEBP2alphaA, were able to elicit additive effects with BMP-4 signaling on Xbr-1a/Xvent-2 reporter activities. These results indicate that transcriptional regulation of the Xbr-1a/Xvent-2 gene occurs in a complex mode through the cooperation of various transcription factors.[1]

References

Transcriptional regulation of Xbr-1a/Xvent-2 homeobox gene: analysis of its promoter region. Lee, H.S., Park, M.J., Lee, S.Y., Hwang, Y.S., Lee, H., Roh, D.H., Kim, J.I., Park, J.B., Lee, J.Y., Kung, H.F., Kim, J. Biochem. Biophys. Res. Commun. (2002) [Pubmed]

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