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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Progression of the stacked Golgi compartments in the yeast Saccharomyces cerevisiae by overproduction of GDP-mannose transporter.

The Golgi compartments of the yeast Saccharomyces cerevisiaeare dispersed within the cytoplasm, in contrast to the stacked cisternae in the mammalian cell, and consequently are observed as a punctate pattern by immunofluorescent staining of Golgi-marker proteins. The VIG4/VRG4 gene encodes the essential yeast GDP-mannose transporter, which is a polytopic membrane protein in the early and medial Golgi compartments. Upon overexpression of this gene by the aid of a strong promoter and multicopy vector, we found that stacked multivesicular structures, which resembled the cisternae of mammalian Golgi apparatus, had developed in S. cerevisiae. Immuno-electron microscopy showed that the GDP-mannose transporter was located on the stacked cisternae. Immuno-isolation and immunoblotting analyses of the vesicles showed that the overproduced GDP-mannose transporter also co-localized with the Golgi glycosyltransferases, but not with the ER- or late Golgi-marker proteins as in the control cell. We propose that the localization mechanism of the GDP-mannose transporter in the Golgi compartment would be efficient and hardly saturable, and therefore the overproduced protein induced a progression of Golgi-like compartments rather than being mislocalized in other compartments, such as the ER or a vacuole.[1]


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