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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Factors involved in concanavalin A agglutination of KB and NC37 cells grown in suspension culture.

NC37 and KB cells grown in suspension culture remained agglutinable at 23 degrees C by concanavalin A (Con A) after sufficient glutaraldehyde fixation to prevent lateral mobility (clustering) of Con A binding sites. In contrast, at 4 degrees C agglutination of the fixed cells was blocked. No significant differences in 3H-Con A binding were observed between unfixed cells and glutaraldehyde-fixed cells at 23 degrees C and unfixed cells at 4 degrees C. Con A bound to fixed cells at 4 degrees C produced agglutination when the cells were washed and then warmed to 23 degrees C. Thus a cold-sensitive factor unrelated to the binding of Con A or the clustering of Con A sites is necessary for agglutination to occur.[1]

References

  1. Factors involved in concanavalin A agglutination of KB and NC37 cells grown in suspension culture. Altenburg, B., Steiner, S. J. Natl. Cancer Inst. (1976) [Pubmed]
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