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MeSH Review

KB Cells

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Disease relevance of KB Cells


High impact information on KB Cells

  • Multi-drug-resistant transfectants obtained with the mutant sequence were characterized by increased relative resistance to colchicine compared with transfectants obtained with wild-type sequence. mdr1 mutations are therefore responsible for preferential resistance to colchicine in multidrug-resistant KB cells [6].
  • A variant form of beta-actin in a mutant of KB cells resistant to cytochalasin B [7].
  • Several KB cell RNAs with long half-lives in vivo, including 5S and bulk 4S RNA, are not cleaved by this nuclease [1].
  • Identification of a ribonuclease P-like activity from human KB cells [1].
  • Expression of a truncated SIF protein resulted in defects in neuronal morphology and induced membrane ruffling with altered actin localization in human KB cells [8].

Chemical compound and disease context of KB Cells

  • To investigate the role of hFR in acquired MTX resistance in human cells, we characterized nine MTX-resistant clones selected from human nasopharyngeal epidermoid carcinoma (KB) cells (cells which transport folates/anti-folates via the hFR) cultured in media containing low folate concentrations [9].
  • Adenovirus type 2 DNA replicated in arginine-starved KB cells is infectious [10].
  • Adenovirus adsorption and sterol redistribution in KB cell plasma membrane [11].
  • Since high extracellular folate (PteGlu) concentrations (EFC) profoundly influenced uptake and toxicity of the anti-PteGlu methotrexate in malignant KB cells, we studied human cells to determine additional mechanisms for PteGlu uptake when the EFC was varied [12].
  • Verapamil, a clinically important calcium channel blocker, has been found to cause a 40-fold enhancement of killing of the human KB cell line by a cytotoxic conjugate of epidermal growth factor with Pseudomonas exotoxin (EGF-PE) [13].

Biological context of KB Cells

  • NC37 and KB cells grown in suspension culture remained agglutinable at 23 degrees C by concanavalin A (Con A) after sufficient glutaraldehyde fixation to prevent lateral mobility (clustering) of Con A binding sites [14].
  • A human homologue (GST1-Hs) of the yeast GST1 gene that encodes a new GTP-binding protein essential for the G1-to-S phase transition of the cell cycle was cloned from the cDNA library of human KB cells [15].
  • Morphologic characterization of the pathway of transferrin endocytosis and recycling in human KB cells [16].
  • The immunoreactive protein was purified to homogeneity and identified as a catalytic subunit of DNA polymerase alpha by molecular mass, by aphidicolin sensitivity, and by recognition by a monoclonal antibody against human KB cell DNA polymerase alpha [17].
  • The role of glycosylation in the biosynthesis and acquisition of ligand-binding activity of the folate-binding protein in cultured KB cells [18].

Anatomical context of KB Cells


Associations of KB Cells with chemical compounds

  • When KB cells which had been passaged in low folate medium were passaged back into either standard medium or low folate medium supplemented with reduced folates, the levels of both folate-binding proteins fell linearly towards the levels in KB cells continuously maintained in standard medium [24].
  • METHODS: Membrane vesicles were prepared from KB-3-1 (clone from parental KB cells) cells and from cisplatin-resistant KCP-4 (a mutant clone derived from KB-3-1 cells) cells [2].
  • These levels significantly increased to 182 +/- 34 and 26 +/- 6 pmol/mg cell protein, respectively, in KB cells serially passaged in low folate medium (containing 2-10 nM 5-methyltetrahydrofolate) [24].
  • Very low accumulation of vincristine or daunorubicin was observed in the multiple-drug-resistant KB cells in comparison with accumulation in the parental KB cells [25].
  • KB cells "pretreated" in culture with the glucocorticoid prednisolone in hyperosmolal medium exhibited a decrease in the levels of the oncoamniotic (FL) isoenzyme and an increase in the Regan isoenzyme [26].

Gene context of KB Cells

  • SKK3 was the only activator of SAPK4 that was induced when KB cells were exposed to a cellular stress or stimulated with interleukin-1 [27].
  • The chimeric proteins bound to the IGF1 receptors of the human lymphoblast IM-9, albeit with reduced affinity, and elicited some of the same biologic effects (increased glucose and amino acid uptake) in human KB cells as did human IGF1, but with reduced specific activity [28].
  • In contrast, hRRM2 was up-regulated by UV in both PC3 cells and KB cells. hRRM2 and p53R2 mRNA levels were assessed by Northern blot, and the results paralleled that of the Western blot [29].
  • We examined vanadate-induced nucleotide trapping in MRP1 stably expressed in KB cell membrane by using 8-azido-[alpha-(32)P]ATP [30].
  • Utilizing a methotrexate-resistant KB cell line designated 1BT, we now report the kinetic basis for altered levels of DHFR RNA observed in FUra-treated cells [31].

Analytical, diagnostic and therapeutic context of KB Cells


  1. Identification of a ribonuclease P-like activity from human KB cells. Koski, R.A., Bothwell, A.L., Altman, S. Cell (1976) [Pubmed]
  2. Adenosine triphosphate-dependent transport of leukotriene C4 by membrane vesicles prepared from cisplatin-resistant human epidermoid carcinoma tumor cells. Fujii, R., Mutoh, M., Sumizawa, T., Chen, Z.S., Yoshimura, A., Akiyama, S. J. Natl. Cancer Inst. (1994) [Pubmed]
  3. A fluorine-containing anthracycline (ME2303) as a new antitumor agent against murine and human tumors and their multidrug-resistant sublines. Tsuruo, T., Yusa, K., Sudo, Y., Takamori, R., Sugimoto, Y. Cancer Res. (1989) [Pubmed]
  4. Initiation of adenovirus DNA replication does not occur via a hairpin mechanism. Sussenbach, J.S., Kujik, M.G. Nucleic Acids Res. (1978) [Pubmed]
  5. Promotion of cell adhesion on fibronectin during adenovirus infection of KB cells. Hashimoto, S., Kumai, H., Nakai, M., Hashimoto, K., Nakatsuji, N. Exp. Cell Res. (1993) [Pubmed]
  6. An altered pattern of cross-resistance in multidrug-resistant human cells results from spontaneous mutations in the mdr1 (P-glycoprotein) gene. Choi, K.H., Chen, C.J., Kriegler, M., Roninson, I.B. Cell (1988) [Pubmed]
  7. A variant form of beta-actin in a mutant of KB cells resistant to cytochalasin B. Toyama, S., Toyama, S. Cell (1984) [Pubmed]
  8. Still life, a protein in synaptic terminals of Drosophila homologous to GDP-GTP exchangers. Sone, M., Hoshino, M., Suzuki, E., Kuroda, S., Kaibuchi, K., Nakagoshi, H., Saigo, K., Nabeshima, Y., Hama, C. Science (1997) [Pubmed]
  9. Decreased expression of the human folate receptor mediates transport-defective methotrexate resistance in KB cells. Saikawa, Y., Knight, C.B., Saikawa, T., Page, S.T., Chabner, B.A., Elwood, P.C. J. Biol. Chem. (1993) [Pubmed]
  10. Adenovirus type 2 DNA replicated in arginine-starved KB cells is infectious. Auborn, K.J., Rouse, H. J. Virol. (1982) [Pubmed]
  11. Adenovirus adsorption and sterol redistribution in KB cell plasma membrane. Hennache, B., Torpier, G., Boulanger, P. Exp. Cell Res. (1982) [Pubmed]
  12. Folate (pteroylglutamate) uptake in human red blood cells, erythroid precursors and KB cells at high extracellular folate concentrations. Evidence against a role for specific folate-binding and transport proteins. Antony, A.C., Kane, M.A., Krishnan, S.R., Kincade, R.S., Verma, R.S. Biochem. J. (1989) [Pubmed]
  13. Verapamil enhances the toxicity of conjugates of epidermal growth factor with Pseudomonas exotoxin and antitransferrin receptor with Pseudomonas exotoxin. Akiyama, S., Gottesman, M.M., Hanover, J.A., Fitzgerald, D.J., Willingham, M.C., Pastan, I. J. Cell. Physiol. (1984) [Pubmed]
  14. Factors involved in concanavalin A agglutination of KB and NC37 cells grown in suspension culture. Altenburg, B., Steiner, S. J. Natl. Cancer Inst. (1976) [Pubmed]
  15. A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells. Hoshino, S., Miyazawa, H., Enomoto, T., Hanaoka, F., Kikuchi, Y., Kikuchi, A., Ui, M. EMBO J. (1989) [Pubmed]
  16. Morphologic characterization of the pathway of transferrin endocytosis and recycling in human KB cells. Willingham, M.C., Hanover, J.A., Dickson, R.B., Pastan, I. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  17. Physical association of the human base-excision repair enzyme uracil DNA glycosylase with the 70,000-dalton catalytic subunit of DNA polymerase alpha. Seal, G., Sirover, M.A. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  18. The role of glycosylation in the biosynthesis and acquisition of ligand-binding activity of the folate-binding protein in cultured KB cells. Luhrs, C.A. Blood (1991) [Pubmed]
  19. Combination iron depletion therapy. Taetle, R., Honeysett, J.M., Bergeron, R. J. Natl. Cancer Inst. (1989) [Pubmed]
  20. Transfection of a glycosylated phosphatidylinositol-anchored folate-binding protein complementary DNA provides cells with the ability to survive in low folate medium. Luhrs, C.A., Raskin, C.A., Durbin, R., Wu, B., Sadasivan, E., McAllister, W., Rothenberg, S.P. J. Clin. Invest. (1992) [Pubmed]
  21. Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport. Goshima, K., Masuda, A., Owaribe, K. J. Cell Biol. (1984) [Pubmed]
  22. Purification and cDNA cloning of SAPKK3, the major activator of RK/p38 in stress- and cytokine-stimulated monocytes and epithelial cells. Cuenda, A., Alonso, G., Morrice, N., Jones, M., Meier, R., Cohen, P., Nebreda, A.R. EMBO J. (1996) [Pubmed]
  23. Involvement of phosphoinositide 3-kinase in insulin- or IGF-1-induced membrane ruffling. Kotani, K., Yonezawa, K., Hara, K., Ueda, H., Kitamura, Y., Sakaue, H., Ando, A., Chavanieu, A., Calas, B., Grigorescu, F. EMBO J. (1994) [Pubmed]
  24. Influence on immunoreactive folate-binding proteins of extracellular folate concentration in cultured human cells. Kane, M.A., Elwood, P.C., Portillo, R.M., Antony, A.C., Najfeld, V., Finley, A., Waxman, S., Kolhouse, J.F. J. Clin. Invest. (1988) [Pubmed]
  25. Circumvention of multiple-drug resistance in human cancer cells by thioridazine, trifluoperazine, and chlorpromazine. Akiyama, S., Shiraishi, N., Kuratomi, Y., Nakagawa, M., Kuwano, M. J. Natl. Cancer Inst. (1986) [Pubmed]
  26. Expression of KB cell alkaline phosphatase isoenzymes during growth in immunosuppressed LEW rats. Singer, R.M., Herz, F. J. Natl. Cancer Inst. (1979) [Pubmed]
  27. Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases. Goedert, M., Cuenda, A., Craxton, M., Jakes, R., Cohen, P. EMBO J. (1997) [Pubmed]
  28. Expression and characterization of an antibody binding specificity joined to insulin-like growth factor 1: potential applications for cellular targeting. Shin, S.U., Morrison, S.L. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  29. The human ribonucleotide reductase subunit hRRM2 complements p53R2 in response to UV-induced DNA repair in cells with mutant p53. Zhou, B., Liu, X., Mo, X., Xue, L., Darwish, D., Qiu, W., Shih, J., Hwu, E.B., Luh, F., Yen, Y. Cancer Res. (2003) [Pubmed]
  30. Nonequivalent nucleotide trapping in the two nucleotide binding folds of the human multidrug resistance protein MRP1. Nagata, K., Nishitani, M., Matsuo, M., Kioka, N., Amachi, T., Ueda, K. J. Biol. Chem. (2000) [Pubmed]
  31. 5-Fluorouracil inhibits dihydrofolate reductase precursor mRNA processing and/or nuclear mRNA stability in methotrexate-resistant KB cells. Will, C.L., Dolnick, B.J. J. Biol. Chem. (1989) [Pubmed]
  32. Human keratinocytes are a source for tumor necrosis factor alpha: evidence for synthesis and release upon stimulation with endotoxin or ultraviolet light. Köck, A., Schwarz, T., Kirnbauer, R., Urbanski, A., Perry, P., Ansel, J.C., Luger, T.A. J. Exp. Med. (1990) [Pubmed]
  33. Purification and identification of a major activator for p38 from osmotically shocked cells. Activation of mitogen-activated protein kinase kinase 6 by osmotic shock, tumor necrosis factor-alpha, and H2O2. Moriguchi, T., Toyoshima, F., Gotoh, Y., Iwamatsu, A., Irie, K., Mori, E., Kuroyanagi, N., Hagiwara, M., Matsumoto, K., Nishida, E. J. Biol. Chem. (1996) [Pubmed]
  34. Molecular cloning and characterization of the human folate-binding protein cDNA from placenta and malignant tissue culture (KB) cells. Elwood, P.C. J. Biol. Chem. (1989) [Pubmed]
  35. Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. Cohen, G.H., Ponce de Leon, M., Diggelmann, H., Lawrence, W.C., Vernon, S.K., Eisenberg, R.J. J. Virol. (1980) [Pubmed]
  36. Purification and preliminary immunological characterization of the type 5 adenovirus, nonstructural 100,000-dalton protein. Oosterom-Dragon, E.A., Ginsberg, H.S. J. Virol. (1980) [Pubmed]
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