Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis.
The efficacy of the Be82 gene product fused with glutathione S-transferase ( GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported previously (H. Hirata et al., J. Clin. Microbiol. 40:1470-1474, 2002). However, the ELISA with the GST/Be82 antigen cross-reacted with Babesia caballi-infected horse sera, despite the high rate of detection of B. equi. These results suggested that GST/Be82 has an antigen in common with B. caballi or antigenicity similar to that of B. caballi. In the present study, we constructed a series of five clones with deletions in the Be82 gene product, each of which was fused with GST, and used them in ELISAs in order to overcome the cross-reactivity seen with B. caballi. One of the deletion clones, a clone with a deletion of the Be82 gene from positions 236 to 381 (Be82/236-381), specifically and sensitively detected B. equi-infected horse sera without cross-reactivity with B. caballi-infected horse sera. Assays with clones from which other gene products were deleted showed decreased sensitivities or remained nonspecific for the detection of B. equi-infected horse sera. These results suggest that the Be82/236-381 gene product is a novel antigen for the diagnosis of B. equi infection in horses.[1]References
- Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis. Hirata, H., Xuan, X., Yokoyama, N., Nishikawa, Y., Fujisaki, K., Suzuki, N., Igarashi, I. J. Clin. Microbiol. (2003) [Pubmed]
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