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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase.

Lipoic Acid Synthase (LipA) can accommodate a [4Fe-4S] cluster that is thought to be essential for the insertion of sulfur into an octanoyl substrate during the biosynthesis of lipoic acid. With the objective of improving soluble holo-LipA expression, a series of multi-cistronic plasmids were constructed carrying lipA in combination with one of the three systems: groE/SL, trxA, or the isc operon. Co-expression of lipA with the isc operon approximately trebled the isolated yield of soluble LipA and resulted in efficient assembly of the Fe-S cluster. This strategy may be helpful in the soluble expression of a wide range of Fe-S cluster-dependent proteins.[1]

References

  1. Effect of iron-sulfur cluster assembly proteins on the expression of Escherichia coli lipoic acid synthase. Kriek, M., Peters, L., Takahashi, Y., Roach, P.L. Protein Expr. Purif. (2003) [Pubmed]
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