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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice.

Inefficient gene transfer, inaccessibility of stem cell compartments, transient gene expression, and adverse immune and inflammatory reactions to vector and transgenic protein are major barriers to successful in vivo application of gene therapy for most genetic diseases. Prenatal gene therapy with integrating vectors may overcome these problems and prevent early irreparable organ damage. To this end, high-dose attenuated VSV-G pseudotyped equine infectious anaemia virus (EIAV) encoding beta-galactosidase under the CMV promoter was injected into the fetal circulation of immuno-competent MF1 mice. We saw prolonged, extensive gene expression in the liver, heart, brain and muscle, and to a lesser extent in the kidney and lung of postnatal mice. Progressive clustered hepatocyte staining suggests clonal expansion of cells stably transduced. We thus provide proof of principle for efficient gene delivery and persistent transgene expression after prenatal application of the EIAV vector and its potential for permanent correction of genetic diseases.[1]

References

  1. Long-term transgene expression by administration of a lentivirus-based vector to the fetal circulation of immuno-competent mice. Waddington, S.N., Mitrophanous, K.A., Ellard, F.M., Buckley, S.M., Nivsarkar, M., Lawrence, L., Cook, H.T., Al-Allaf, F., Bigger, B., Kingsman, S.M., Coutelle, C., Themis, M. Gene Ther. (2003) [Pubmed]
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