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Noda, K. et al.

Noda, Watanabe, Maruhashi,

Cloning of a rhodococcal promoter using a transposon for dibenzothiophene biodesulfurization.

The expression of biodesulfurization genes (dsz) in Rhodococcus erythropolis strain KA2-5-1 is repressed by sulfate which is the product of biodesulfurization. The application of a sulfate non-repressible promoter could be effective in enhancing biodesulfurization. A promoter-probe transposon was constructed using the promoterless, red-shifted green fluorescence protein gene (rsgfp). A 340 bp putative promoter element, designated kap1, was isolated from a strain KA2-5-1 recombinant that had shown high fluorescence intensity. The activity of kap1 was not affected by 1 mM sulfate. It gave about a 2-fold greater activity than the 16S ribosomal RNA promoter in R. erythropolis strain KA2-5-1 and is therefore useful for expressing desulfurization genes in rhodococcal strains.[1]

References

Cloning of a rhodococcal promoter using a transposon for dibenzothiophene biodesulfurization. Noda, K., Watanabe, K., Maruhashi, K. Biotechnol. Lett. (2003) [Pubmed]

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