A rapid and strong increase of plasminogen activator induced by experimental anaphylaxis in rabbits.
Anaphylactic shock was induced in rabbits by injecting bovine serum albumin ( BSA) as an antigen. Measurements of the enzyme activities in the fibrinolytic system confirmed that a rapid and strong increase of plasminogen activator (PA) was induced during anaphylaxis. The euglobulin fibrinolytic activity (EFA) as estimated by the plasminogen-rich fibrin plate method rose significantly, peaking at 15 min after the BSA injection (when the arterial pressure was minimum). However, EFA was not detected by the plasminogen-poor fibrin plate method. The tissue-type PA (t-PA) activity using the natural substrate plasminogen increased significantly with a peak at 15 min. The amidolytic activity also simultaneously increased significantly using the t-PA substrate, H-D-Ile-Pro-Arg-pNA. The plasminogen activator inhibitor ( PAI) activity remained at baseline levels until 30 min, but rose fourfold at 90 min. The main plasma fibrinolytic enzyme which increased in anaphylaxis was proved by zymography to be t-PA with a molecular weight (MW) of 69,000.[1]References
- A rapid and strong increase of plasminogen activator induced by experimental anaphylaxis in rabbits. Shimaya, K., Sumi, H., Maruyama, M., Mihara, H. Enzyme (1992) [Pubmed]
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