Vitamin K-dependent gamma-carboxylation of the 1,25-dihydroxyvitamin D3 receptor.
It has been reported that vitamin K deficiency in the rat markedly increases the 1,25-dihydroxyvitamin D3 receptor ( VDR) binding to DNA and that vitamin K-dependent gamma-carboxylation of endogenous substrates of the intestinal and renal cytosol, also containing VDR, sharply reduced that binding (Sergeev, I.N., and Spirichev, V.B. (1989) Nutr. Res. 9, 725-733). In the present study we have evaluated vitamin K-dependent 14CO2 incorporation to VDR quantitated by immunoprecipitation with anti-VDR monoclonal antibodies. The results obtained strongly suggest that VDR in vitro can undergo gamma-carboxylation in the presence of vitamin K1 and that 15-25% of Glu residues in the VDR are carboxylated in vivo. Taking into account our earlier findings, it is likely that the VDR gamma-carboxylation modulates its binding to DNA.[1]References
- Vitamin K-dependent gamma-carboxylation of the 1,25-dihydroxyvitamin D3 receptor. Sergeev, I.N., Norman, A.W. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
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