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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Structure and chromosomal localization of the human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene.

Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI mRNA. Human CNPase cDNAs were used to isolate genomic clones containing the human CNPase gene which is 9 kb long. Four exons were identified, separated by three introns, and the sequence of each exon and intron/exon boundary has been established. The polymerase chain reaction (PCR) was used to detect the presence of the human CNPase gene in DNA from a panel of rodent/human somatic cell hybrids. By this means the human CNPase gene was mapped to chromosome 17. In situ hybridization of a human CNPase genomic clone to metaphase chromosomes further localized this gene to chromosomal band 17q21.[1]

References

  1. Structure and chromosomal localization of the human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene. Douglas, A.J., Fox, M.F., Abbott, C.M., Hinks, L.J., Sharpe, G., Povey, S., Thompson, R.J. Ann. Hum. Genet. (1992) [Pubmed]
 
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