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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae.

A simple method is described for the direct isolation of zymosterol (5 alpha-cholesta-8,24-dien-3 beta-ol) of high purity from a sterol mutant of Saccharomyces cerevisiae. This yeast strain, which is a double mutant of the ERG6 (sterol transmethylase) and ERG2 (C-8 sterol isomerase) genes, accumulates zymosterol as its major sterol component.[1]

References

  1. A simple method for the isolation of zymosterol from a sterol mutant of Saccharomyces cerevisiae. Heiderpriem, R.W., Livant, P.D., Parish, E.J., Barbuch, R.J., Broaddus, M.G., Bard, M. J. Steroid Biochem. Mol. Biol. (1992) [Pubmed]
 
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