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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Determination of butein in rat serum by high performance liquid chromatography.

A simple high performance liquid chromatography (HPLC) method was developed for the determination of butein in rat serum. The method involved the deproteinization followed by injection into a Luna C8 column. Butein was eluted at 3.8 min at a flow rate of 0.2 ml/min with the mobile phase of acetonitrile-ammonium formate (10mM, pH 3.0) (35:65, v/v). The standard curve was linear (r(2)=0.995) over the concentration range of 0.1-10 microg/ml. The coefficient of variation (CV) of intra- and inter-assay ranged from 2.7 to 7.5% and 6.0 to 7.5%, respectively. The limit of quantification was 0.1 microg/ml using a serum sample of 50 microl. This method was applied to a pharmacokinetic study after intravenous injection of butein (5mg/kg) to rats.[1]


  1. Determination of butein in rat serum by high performance liquid chromatography. Lee, H.W., Ji, H.Y., Lee, H.I., Kim, H.K., Sohn, D.H., Kim, Y.C., Chung, H.T., Lee, H.S. Journal of pharmaceutical and biomedical analysis. (2004) [Pubmed]
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