Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis.
Second mitochondria-derived activator of caspase ( Smac) has been implicated in the activation of apoptosis in response to cell stress. We screened for Smac/ DIABLO-binding protein for further understanding of Smac-mediated apoptosis. We identified NADE, previously known as p75NTR-associated cell death executor, as a Smac-binding protein. Smac-NADE interaction was mapped to the N-terminal region of Samc and the C-terminal region of NADE. Co-expression of NADE and Smac promotes TRAIL-induced apoptosis in MCF-7 cells. Interestingly, the co-presence of Smac and NADE inhibits XIAP- mediated Smac ubiquitination. In conclusion, our results provide the first evidence that the interaction between Smac and NADE regulates apoptosis through the inhibition of Smac ubiquitination.[1]References
- Direct interaction of Smac with NADE promotes TRAIL-induced apoptosis. Yoon, K., Jang, H.D., Lee, S.Y. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
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