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Determination of liquiritigenin and isoliquiritigenin in Glycyrrhiza uralensis and its medicinal preparations by capillary electrophoresis with electrochemical detection.

A simple, reliable, reproducible and sensitive method, based on capillary electrophoresis with electrochemical detection (ED), for the determination of liquiritigenin and isoliquiritigenin in Glycyrrhiza uralensis and its medicinal preparations was described. Operated in a wall-jet configuration, a 300 microm diameter carbon-disk electrode was used as the working electrode, which exhibits good responses at + 1000 mV (versus SCE) for the two analytes. Under the optimum conditions, the analytes were base-line separated within 8 min, and excellent linearity was obtained in the concentration range from 5.0 x 10(-4) to 1.0 x 10(-6) mol/l. The detection limit (S/N = 3) was 4.7 x 10(-7) and 2.9 x 10(-7) mol/l for liquiritigenin and isoliquiritigenin, respectively. This work provides a useful method for the analysis of traditional Chinese medicines.[1]

References

  1. Determination of liquiritigenin and isoliquiritigenin in Glycyrrhiza uralensis and its medicinal preparations by capillary electrophoresis with electrochemical detection. Cao, Y., Wang, Y., Ji, C., Ye, J. Journal of chromatography. A. (2004) [Pubmed]
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