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Crystallization of microsomal triglyceride transfer protein from bovine liver.

The microsomal triglyceride transfer protein ( MTP) is a heterodimeric lipid transfer protein required for the assembly of plasma very low density lipoproteins in the liver and chylomicrons in the intestine. Bovine MTP was purified by a modification of a previously published procedure and crystals of MTP were grown reproducibly with polyethylene glycol as a precipitant at pH 7. 0. MTP crystals, which diffract to Bragg spacings of better than 3.2 A, have the symmetry of space group P2(1)2(1)2(1) with refined lattice constants of a = 88.7, b = 100.9 and c = 201.1 A, with one heterodimer per asymmetric unit.[1]

References

  1. Crystallization of microsomal triglyceride transfer protein from bovine liver. Ohringer, S.L., Jamil, H., Chu, C.H., Tabernero, L., Einspahr, H.M., Wetterau, J.R., Sack, J.S. Acta Crystallogr. D Biol. Crystallogr. (1996) [Pubmed]
 
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