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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Structural basis of endothelial lipase tropism for HDL.

Lipoprotein lipase ( LPL) and endothelial lipase ( EL), the most closely related enzymes among the members of the triglyceride lipase gene family with regard to primary sequence, have distinct lipolytic properties (triglyceride lipase vs. phospholipase) as well as different preferences for specific types of lipoproteins [triglyceride-rich lipoproteins vs. high density lipoprotein (HDL)] Lipid substrate specificity is believed to be conferred by the lid region located in the amino-terminal domain of the enzymes, whereas surprisingly little work has been done to identify the region mediating lipoprotein substrate specificity. To determine the domain responsible for lipoprotein preference within each enzyme, we generated the domain chimeric enzyme LPL- EL. The heterologous carboxy-terminal (C terminal) domain did not change lipid substrate preference (triglyceride vs. phospholipase) as determined by using artificial substrates. The EL C-terminal domain, however, enabled LPL- EL to bridge HDL particles like wild-type EL, whereas LPL only mediated binding of very low density lipoprotein. Unlike wild-type LPL, LPL- EL had substantial ability to hydrolyze HDL lipids similar to that of wild-type EL. Overexpression of LPL- EL in wild-type mice resulted in significantly reduced levels of HDL cholesterol and phospholipids by 93 and 85%, respectively, similar to the extent seen in EL-expressing mice, whereas no reduction of these parameters was observed in LPL-expressing mice. We conclude that the C-terminal domain of EL is crucial for the ability of EL to bind and to hydrolyze HDL and converts LPL to an enzyme fully capable of hydrolyzing HDL, highlighting the importance of the C-terminal lipase domain in lipoprotein substrate preference.[1]


  1. Structural basis of endothelial lipase tropism for HDL. Broedl, U.C., Jin, W., Fuki, I.V., Glick, J.M., Rader, D.J. FASEB J. (2004) [Pubmed]
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